Detection of brain-derived neurotrophic factor-like activity in fibroblasts and Schwann cells: inhibition by antibodies to NGF
| Autor: | Ralph F. Alderson, RA Murphy, Ann Acheson, Philip A. Barker, Freda D. Miller |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Cell Survival
Blotting Western Schwann cell Nerve Tissue Proteins Chick Embryo Biology Antibodies Mice Neurotrophic factors medicine Animals Nerve Growth Factors Neurons Afferent RNA Messenger Fibroblast Cells Cultured Brain-derived neurotrophic factor General Neuroscience Brain-Derived Neurotrophic Factor Brain Fibroblasts Sensory neuron Axons Cell biology Culture Media Rats medicine.anatomical_structure Nerve growth factor nervous system Cell culture Immunology Neuroglia Schwann Cells |
| Zdroj: | Neuron. 7(2) |
| ISSN: | 0896-6273 |
| Popis: | mRNA coding for brain-derived neurotrophic factor (BDNF) has been detected in cultured L929 fibroblasts, rat dermal fibroblasts, and sciatic nerve Schwann cells, as well as in rat skin. Medium conditioned by cultured fibroblasts and Schwann cells also stimulates neurite growth from retinal explants and promotes the survival in culture of BDNF-responsive sensory neurons; biological activity is abolished by antibodies raised against NGF. These results suggest that molecules with BDNF-like activity may be produced by cells in the peripheral nervous system and that the BDNF-like activity in fibroblasts and Schwann cells is derived from molecules immunologically related to NGF. In support of this concept, antibodies against NGF have been found to reduce the biological activity of recombinant BDNF in culture and to cross-react with BDNF on Western blots. |
| Databáze: | OpenAIRE |
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