Synthesis of a tetratriacontapeptide corresponding to sequence 112-145 of the β-subunit of hCG and production of antiserum against this synthetic peptide
| Autor: | Masami Yagyu, Shin Iguchi, Osamu Tanizawa, Nagatoshi Sugita, Kenji Yamaji, Koichi Kawasaki, T. Takagi, Yoshio Okada |
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| Rok vydání: | 2010 |
| Předmět: |
endocrine system
Immunogen Radioimmunoassay Sequence (biology) Peptide Chorionic Gonadotropin chemistry.chemical_compound Animals Chorionic Gonadotropin beta Subunit Human Bovine serum albumin reproductive and urinary physiology Carbodiimide chemistry.chemical_classification Antiserum biology urogenital system Chemistry Obstetrics and Gynecology Molecular biology Peptide Fragments biology.protein Cattle Rabbits Glutaraldehyde hormones hormone substitutes and hormone antagonists |
| Zdroj: | Asia-Oceania Journal of Obstetrics and Gynaecology. 8:291-296 |
| ISSN: | 0389-2328 |
| DOI: | 10.1111/j.1447-0756.1982.tb00579.x |
| Popis: | A tetratriacontapeptide(P-34) corresponding to sequence 112–145 of the β-subunit of hCG(hCG-β) was synthesized by the fragment condensation method. The immunogen was prepared by coupling P-34 to bovine serum albumin by the carbodiimide or glutaraldehyde method. Antiserum was produced by injecting the immunogen into rabbits at multiple intradermal and intrasplenal sites with Freund's complete adjuvant. Eight weeks after the first immunization, antiserum was obtained that bound 30% of 125I-hCG or 125I-hCG-β at a dilution of 1:1,000. This binding was inhibited dose-dependently by hCG. In this radioimmunoassay system, the minimal detectable dose of hCG was 500 mIU/ml and, as expected theoretically, no binding to 125I-hCG-α or 125I-hLH was observed. |
| Databáze: | OpenAIRE |
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