Cell Fusion of Mesenchymal Stem/Stromal Cells and Breast Cancer Cells Leads to the Formation of Hybrid Cells Exhibiting Diverse and Individual (Stem Cell) Characteristics
| Autor: | Ola Myklebost, Jessica Dörnen, Thomas Dittmar |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
cancer stem cells
Epithelial-Mesenchymal Transition Stromal cell Apoptosis Breast Neoplasms Hybrid Cells Article Catalysis lcsh:Chemistry Inorganic Chemistry breast cancer Cell Movement Cancer stem cell Tumor Cells Cultured Humans Epithelial–mesenchymal transition Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Cell Proliferation cell fusion Cell fusion biology hybrid clones Organic Chemistry Mesenchymal stem cell CD44 EMT Mesenchymal Stem Cells General Medicine Computer Science Applications Gene Expression Regulation Neoplastic lcsh:Biology (General) lcsh:QD1-999 Cancer cell Neoplastic Stem Cells Cancer research biology.protein Female Stem cell |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 24 International Journal of Molecular Sciences, Vol 21, Iss 9636, p 9636 (2020) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms21249636 |
| Popis: | Cancer is one of the most common diseases worldwide, and treatment bears many challenges such as drug and radioresistance and formation of metastases. These difficulties are due to tumor heterogeneity, which has many origins. One may be cell fusion, a process that is relevant in both physiological (e.g., wound healing) and pathophysiological (cancer and viral infection) processes. In this study, we examined if cell fusion between mesenchymal stem/stromal cells (MSCs) and breast cancer (BC) cells occurs and if newly generated hybrid cells may exhibit cancer stem/initiating cell (CS/IC) characteristics. Therefore, several methods such as mammosphere assay, AldeRed assay, flow cytometry (CD24, CD44, CD104) and Western blot analysis (of epithelial to mesenchymal transition markers such as SNAIL, SLUG and Twist) were applied. In short, four different hybrid clones, verified by short tandem repeat (STR) analysis, were analyzed each expressed an individual phenotype that seemed not to be explicitly related to either a more stem cell or cancer cell phenotype. These results show that cancer cells and MSCs are able to fuse spontaneously in vitro, thereby giving rise to hybrid cells with new properties, which likely indicate that cell fusion may be a trigger for tumor heterogeneity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |