| Zdroj: |
Niku-Paavola, M-L, Fagerström, R, Kruus, K & Viikari, L 2004, ' Thermostable laccases produbed by a white-rot fungus from Peniophora species ', Enzyme and Microbial Technology, vol. 35, no. 1, pp. 100-102 . https://doi.org/10.1016/j.enzmictec.2004.03.008 |
| Popis: |
A strain of Peniophora species was shown to produce laccase, which is a new feature for this species. Peniophora secreted several laccase isoforms with pI-values 3.7–4.2 when grown on glucose and soya meal medium. The Peniophora laccases were typical for Basidiomycetes with respect to pH-dependence, specific activity and inhibitors. The interesting exceptional feature of Peniophora laccases was their good thermostability. At 60 °C, the half-life of the isoforms in the culture filtrate was approximately 5 h and at 70 °C for 15 min. Also, the isolated main isoform with pI-value 4.1 and molecular mass 63 kDa was similarly thermostable. The N-terminal amino acid sequence, altogether 22 amino acid residues, of the separated isoform was 50–63% identical with those of the Basidiomycete laccases analyzed recently. |
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