Heme oxygenase is the main protective enzyme in rat liver upon 6-day administration of cobalt chloride
| Autor: | Anna Braykova, Milka S. Setchenska, Thomas B. Bolton, Duridanova Db, Tania Y Christova |
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| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Bilirubin Health Toxicology and Mutagenesis Injections Subcutaneous Glutathione reductase Toxicology medicine.disease_cause Lipid peroxidation Superoxide dismutase chemistry.chemical_compound Internal medicine medicine Animals Rats Wistar chemistry.chemical_classification biology Dose-Response Relationship Drug Glutathione peroxidase General Medicine Glutathione Cobalt Rats Heme oxygenase Enzyme Activation Oxidative Stress Endocrinology chemistry Biochemistry Heme Oxygenase (Decyclizing) biology.protein Microsomes Liver Lipid Peroxidation Oxidative stress |
| Zdroj: | Archives of toxicology. 75(8) |
| ISSN: | 0340-5761 |
| Popis: | Changes in the activities of rat liver heme oxygenase (HO), superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and glutathione reductase (GR), as well as changes in lipid peroxidation and reduced glutathione (GSH) levels were measured after acute loading and chronic administration of cobalt chloride (CoCl2). Acute loading was achieved by a single subcutaneous injection of 60 mg CoCl2/kg body weight for 24 h. Chronic administration was performed by giving the same total amount of CoCl2 in small doses over longer periods of time: 30 mg CoCl2/kg daily for 2 days, 15 mg CoCl2/kg daily for 4 days, or 10 mg CoCl2/kg daily for 6 days. The results showed that HO activity was increased both after acute loading (7-fold increase) and upon 6-day administration of CoCl2 (5-fold increase). The GSH level, 24 h after a single injection of CoCl2, was lower than that of the control animals. However, upon chronic administration of small doses CoCl2, the level of GSH increased and was accompanied by an increase in GR activity. Chronic administration of CoCl2 produced persistent oxidative stress, which was illustrated with a continuous increase in lipid peroxidation. At the same time, under these conditions, the activities of oxidative-stress-protective enzymes were either inhibited (SOD, catalase) or not significantly changed (GPx). Collectively, these findings suggest that the sustained up-regulation of HO activity in rat liver upon 6 day administration of CoCl2 would be beneficial by providing the cells with antioxidants, biliverdin and bilirubin, and together with the increased levels of GSH would act as a part of the defence mechanisms against the cobalt-induced oxidative stress. |
| Databáze: | OpenAIRE |
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