Expansive spread of IncI1 plasmids carrying blaCMY-2 amongst Escherichia coli
Autor: | Kwee Yong Seah, Anna L. Sartor, Graeme R. Nimmo, Claire Heney, Joan Faoagali, David L. Paterson, Lyndall Coleman, Hanna E. Sidjabat, Petra Derrington |
---|---|
Rok vydání: | 2013 |
Předmět: |
Microbiology (medical)
Salmonella Gene Transfer Horizontal Biology medicine.disease_cause Synteny beta-Lactamases Microbiology Plasmid Gene Order medicine Escherichia coli Humans Pharmacology (medical) Replicon Typing Insertion sequence Escherichia coli Infections Phylogeny Genetics Phylogenetic tree General Medicine Sequence Analysis DNA Molecular Typing Infectious Diseases Horizontal gene transfer Urinary Tract Infections DNA Transposable Elements Plasmids |
Zdroj: | International journal of antimicrobial agents. 44(3) |
ISSN: | 1872-7913 |
Popis: | Escherichia coli is a leading cause of urinary tract infections. One of the most common antibiotic classes used to treat such infections is the β-lactams, including cephalosporins. Resistance to the third-generation cephalosporins can be caused by production of extended-spectrum β-lactamases (ESBLs) or plasmid-mediated AmpC β-lactamases. The most commonly reported AmpC β-lactamase in E. coli is CMY-2. Plasmid-mediated CMY-2 has been frequently reported in E. coli and Salmonella sp. from food-producing animals. This study aimed to elucidate the molecular characteristics of clinical E. coli isolates carrying plasmids encoding CMY-2. A total of 67 CMY-2-producing E. coli were characterised by clonal analysis and phylogenetic typing. Characterisation of the plasmids carrying blaCMY-2 included replicon typing, plasmid profiling, plasmid transferability and sequencing of the blaCMY-2 genetic environment. As a result, E. coli producing CMY-2 was found to be highly polyclonal. The majority of CMY-2-producing E. coli belonged to phylogenetic group D. IncI1 plasmids were predominant among those carrying blaCMY-2 (96%). Restriction analysis revealed a single IncI1 plasmid carrying blaCMY-2 to be predominant and present in different clones of E. coli. IS1294-ISEcp1 complex or ISEcp1 that was truncated by IS1294 was the predominant insertion sequence upstream of blaCMY-2. The homogeneous genetic environment of blaCMY-2 observed among different strains of E. coli strongly suggests horizontal transfer of this IncI1, blaCMY-2-carrying plasmid. In summary, horizontal plasmid transfer plays a major role in the spread of blaCMY-2 in E. coli. |
Databáze: | OpenAIRE |
Externí odkaz: |