Electrospray Analysis of Biological Samples for Trace Amounts of Trichloroacetic Acid, Dichloroacetic Acid, and Monochloroacetic Acid
| Autor: | Christel T. Bishop, Wayne T. Brashear, Richat Abbas |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Detection limit
chemistry.chemical_classification Chemical Health and Safety Chromatography Dichloroacetic Acid Health Toxicology and Mutagenesis Metabolite Electrospray ionization Carboxylic acid Dichloroacetic acid Acetates Reference Standards Toxicology Mass spectrometry Sensitivity and Specificity Mass Spectrometry Analytical Chemistry chemistry.chemical_compound chemistry Administration Inhalation Humans Environmental Chemistry Trichloroacetic Acid Trichloroacetic acid Quantitative analysis (chemistry) |
| Zdroj: | Journal of Analytical Toxicology. 21:330-334 |
| ISSN: | 1945-2403 0146-4760 |
| DOI: | 10.1093/jat/21.5.330 |
| Popis: | Trichloroethylene (TCE) has been identified as a widespread groundwater contaminant. Trichloroacetic acid (TCA) and dichloroacetic acid (DCA) are toxicologically relevant metabolites of TCE that produce tumors in B6C3F1 mice. A sensitive method for measuring these metabolites in plasma has been developed to obtain pharmacokinetic data from TCE exposure. This is particularly important because DCA is more potent at producing hepatoproliferative lesions than TCA. At present, it is unclear whether DCA is produced by humans. Existing gas chromatographic methods cannot detect DCA at low nanogram-per-milliliter levels. A Finnigan TSQ 700 mass spectrometer (MS) with electrospray ionization was used to measure TCA, DCA, and monochloroacetic acid (MCA) in plasma. The MS was operated in negative ion tandem MS mode. The limit of detection for TCA and DCA was 4 ng/ml, and the limit of detection for MCA was 25 ng/mL. Plasma samples from human subjects exposed to 100 ppm TCE for 4 h contained TCA at concentrations as high as 10 micrograms/mL. DCA concentrations were less than 5 ng/mL, and MCA was not detected (less than 25 ng/mL). |
| Databáze: | OpenAIRE |
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