Diversity of A(H5N1) clade 2.3.2.1c avian influenza viruses with evidence of reassortment in Cambodia, 2014-2016
| Autor: | Matthew Kaye, Merryn Roe, Erik A. Karlsson, Paul F. Horwood, Sokhoun Yann, San Sorn, Davun Holl, Aeron C. Hurt, Annika Suttie, Dhanasekaran Vijaykrishna, Songha Tok, Philippe Buchy, Ponnarath Keo, Srey Viseth Horm, Philippe Dussart, Ian G. Barr, Andrew R. Greenhill, Sothyra Tum, Yi Mo Deng |
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| Přispěvatelé: | Unité de Virologie / Virology Unit [Phnom Penh], Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Federation University [Churchill, Australia], The Peter Doherty Institute for Infection and Immunity [Melbourne], The Royal Melbourne Hospital-University of Melbourne, Ministry of Agriculture, Forestry and Fisheries [Cambodia], GlaxoSmithKline Vaccines [Singapore], GlaxoSmithKline [Headquarters, London, UK] (GSK), Monash University [Melbourne], James Cook University (JCU), This publication is the result of work conducted under a cooperative agreement with the Office of the Assistant Secretary for Preparedness and Response in the U.S. Department of Health and Human Services (HHS), grant number IDSEP140020-01-00. Its contents and conclusions are solely the responsibility of the authors and do not represent the official views of HHS. The study was also funded, in part, by the US Agency for International Development (grant No. AID-442-G-14-00005) and partially funded through the UK Research and Innovation Global Challenges Research Fund to The Consortium of Animal Market Networks to Assess Risk of Emerging Infectious Diseases Through Enhanced Surveillance (CANARIES, grant No. GCRFNGR3\1497). Annika Suttie is funded by an Australian Government Research Training Program Scholarship and a Faculty of Science and Technology Research Scholarship from Federation University. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health. GlaxoSmithKline Biologicals SA provided support in the form of salary for an author [PB], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific role of this author is articulated in the ‘author contributions’ section. The authors are solely responsible for final content and interpretation. |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Epidemiology MESH: Selection Genetic viruses animal diseases Reassortment MESH: Virulence Pathology and Laboratory Medicine [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy medicine.disease_cause Poultry Geographical Locations MESH: Genotype Medicine and Health Sciences Influenza A virus MESH: Animals MESH: Genetic Variation MESH: Phylogeny Clade Phylogeny Data Management Viral Genomics Multidisciplinary Virulence biology Microbial Mutation MESH: Chickens Eukaryota virus diseases Phylogenetic Analysis Genomics 3. Good health Phylogenetics MESH: Hemagglutinins Hemagglutinins Infectious Diseases MESH: Reassortant Viruses Medical Microbiology Viral Pathogens Vertebrates Viruses [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Medicine Pathogens Cambodia Reassortant Viruses Research Article medicine.drug Computer and Information Sciences Asia Genotype Infectious Disease Control MESH: Influenza A Virus H5N1 Subtype Science MESH: Bayes Theorem 030106 microbiology Hemagglutinin (influenza) Microbial Genomics Disease Surveillance MESH: Poultry Diseases Microbiology Birds 03 medical and health sciences Zanamivir MESH: Influenza in Birds Virology Genetics medicine Animals Evolutionary Systematics Selection Genetic Microbial Pathogens Poultry Diseases Taxonomy Evolutionary Biology Influenza A Virus H5N1 Subtype Population Biology MESH: Cambodia Organisms Genetic Variation Biology and Life Sciences Bayes Theorem Influenza A virus subtype H5N1 030104 developmental biology Influenza in Birds Infectious Disease Surveillance Amniotes People and Places biology.protein Peramivir Chickens Neuraminidase Population Genetics |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2019, 14 (12), pp.e0226108. ⟨10.1371/journal.pone.0226108⟩ PLoS ONE, Vol 14, Iss 12, p e0226108 (2019) |
| ISSN: | 1932-6203 |
| Popis: | International audience; In Cambodia, highly pathogenic avian influenza A(H5N1) subtype viruses circulate endemically causing poultry outbreaks and zoonotic human cases. To investigate the genomic diversity and development of endemicity of the predominantly circulating clade 2.3.2.1c A (H5N1) viruses, we characterised 68 AIVs detected in poultry, the environment and from a single human A(H5N1) case from January 2014 to December 2016. Full genomes were generated for 42 A(H5N1) viruses. Phylogenetic analysis shows that five clade 2.3.2.1c genotypes, designated KH1 to KH5, were circulating in Cambodia during this period. The genotypes arose through multiple reassortment events with the neuraminidase (NA) and internal genes belonging to H5N1 clade 2.3.2.1a, clade 2.3.2.1b or A(H9N2) lineages. Phylogenies suggest that the Cambodian AIVs were derived from viruses circulating between Cambodian and Vietnamese poultry. Molecular analyses show that these viruses contained the hemagglutinin (HA) gene substitutions D94N, S133A, S155N, T156A, T188I and K189R known to increase binding to the human-type α2,6-linked sialic acid receptors. Two A (H5N1) viruses displayed the M2 gene S31N or A30T substitutions indicative of adamantane resistance, however, susceptibility testing towards neuraminidase inhibitors (oseltamivir, zanamivir, lananmivir and peramivir) of a subset of thirty clade 2.3.2.1c viruses showed susceptibility to all four drugs. This study shows that A(H5N1) viruses continue to reassort with other A(H5N1) and A(H9N2) viruses that are endemic in the region, highlighting the risk of introduction and emergence of novel A(H5N1) genotypes in Cambodia. |
| Databáze: | OpenAIRE |
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