Expression of Specific Alleles of Zinc-Finger Transcription Factors, HvSAP8 and HvSAP16, and Corresponding SNP Markers, Are Associated with Drought Tolerance in Barley Populations
| Autor: | Peter A. Anderson, Carly Schramm, Satyvaldy Jatayev, Sergey Sereda, Peter Langridge, Colin L. D. Jenkins, Sunita Gupta, Bekzak Amantayev, N. K. Gupta, Akmaral Baidyussen, Yuri Shavrukov, Grigory Sereda, Gulmira Khassanova, Kathleen L. Soole |
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| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
Drought tolerance Single-nucleotide polymorphism drought zinc-finger transcription factors Biology Catalysis SNP plant genotyping Inorganic Chemistry Gene expression Genotype SNP grain yield and yield components Biology (General) Physical and Theoretical Chemistry Allele QD1-999 Molecular Biology Gene Spectroscopy Genetics thousand grain weight fungi Organic Chemistry ASQ and Amplifluor markers barley food and beverages General Medicine Computer Science Applications stress-associated genes shoots number per plant Chemistry Shoot gene expression |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 12156, p 12156 (2021) International Journal of Molecular Sciences Volume 22 Issue 22 |
| ISSN: | 1422-0067 |
| Popis: | Two genes, HvSAP8 and HvSAP16, encoding Zinc-finger proteins, were identified earlier as active in barley plants. Based on bioinformatics and sequencing analysis, six SNPs were found in the promoter regions of HvSAP8 and one in HvSAP16, among parents of two barley segregating populations, Granal × Baisheshek and Natali × Auksiniai-2. ASQ and Amplifluor markers were developed for HvSAP8 and HvSAP16, one SNP in each gene, and in each of two populations, showing simple Mendelian segregation. Plants of F6 selected breeding lines and parents were evaluated in a soil-based drought screen, revealing differential expression of HvSAP8 and HvSAP16 corresponding with the stress. After almost doubling expression during the early stages of stress, HvSAP8 returned to pre-stress level or was strongly down-regulated in plants with Granal or Baisheshek genotypes, respectively. For HvSAP16 under drought conditions, a high expression level was followed by either a return to original levels or strong down-regulation in plants with Natali or Auksiniai-2 genotypes, respectively. Grain yield in the same breeding lines and parents grown under moderate drought was strongly associated with their HvSAP8 and HvSAP16 genotypes. Additionally, Granal and Natali genotypes with specific alleles at HvSAP8 and HvSAP16 were associated with improved performance under drought via higher 1000 grain weight and more shoots per plant, respectively. |
| Databáze: | OpenAIRE |
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