SCALLOPED Interacts with YORKIE, the Nuclear Effector of the Hippo Tumor-Suppressor Pathway in Drosophila
| Autor: | Youlian Goulev, Jean Daniel Fauny, Beatriz Gonzalez-Marti, Joël Silber, Alain Zider, Domenico Flagiello |
|---|---|
| Přispěvatelé: | Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), the Association Française contre les Myopathies, the Association pour la Recherche sur le Cancer |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
animal structures
Mutant DEVBIO Protein Serine-Threonine Kinases Biology General Biochemistry Genetics and Molecular Biology law.invention 03 medical and health sciences 0302 clinical medicine Transcription (biology) law Morphogenesis Animals Drosophila Proteins Humans Wings Animal Transcription factor Cell Proliferation 030304 developmental biology Genetics 0303 health sciences Hippo signaling pathway Agricultural and Biological Sciences(all) Effector Schneider 2 cells Biochemistry Genetics and Molecular Biology(all) Tumor Suppressor Proteins fungi Intracellular Signaling Peptides and Proteins Nuclear Proteins YAP-Signaling Proteins [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell biology body regions SIGNALING Trans-Activators Suppressor Drosophila General Agricultural and Biological Sciences Protein Kinases 030217 neurology & neurosurgery Nuclear localization sequence HeLa Cells Signal Transduction Transcription Factors |
| Zdroj: | Current Biology-CB Current Biology-CB, Elsevier, 2008, epub ahead of print. ⟨10.1016/j.cub.2008.02.034⟩ |
| ISSN: | 0960-9822 1879-0445 |
| Popis: | International audience; In Drosophila, SCALLOPED (SD) belongs to a family of evolutionarily conserved proteins characterized by the presence of a TEA/ATTS DNA-binding domain [1, 2]. SD physically interacts with the product of the vestigial (vg) gene, where the dimer functions as a master gene controlling wing formation [3, 4]. The VG-SD dimer activates the transcription of several specific wing genes, including sd and vg themselves [5, 6]. The dimer drives cell-cycle progression by inducing expression of the dE2F1 transcription factor [7], which regulates genes involved in DNA replication and cell-cycle progression. Recently, YORKIE (YKI) was identified as a transcriptional coactivator that is the downstream effector of the Hippo signaling pathway, which controls cell proliferation and apoptosis in Drosophila[8]. We identified SD as a partner for YKI. We show that interaction between YKI and SD increases SD transcriptional activity both ex vivo in Drosophila S2 cells and in vivo in Drosophila wing discs and promotes YKI nuclear localization. We also show that YKI overexpression induces vg and dE2F1 expression and that proliferation induced by YKI or by a dominant-negative form of FAT in wing disc is significantly reduced in a sd hypomorphic mutant context. Contrary to YKI, SD is not required in all imaginal tissues. This indicates that YKI-SD interaction acts in a tissue-specific fashion and that other YKI partners must exist. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|