Fetal wound healing using a genetically modified murine model: the contribution of P-selectin
| Autor: | Bindi Naik-Mathuria, Andre N. Gay, Ling Yu, Jean E. Hsu, C. Wayne Smith, Oluyinka O. Olutoye |
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| Rok vydání: | 2008 |
| Předmět: |
Keratinocytes
Pathology medicine.medical_specialty P-selectin Adhesion (medicine) Scars Inflammation Article Animals Genetically Modified Cicatrix Mice Fibrosis Medicine Animals Mice Knockout Fetus Wound Healing integumentary system business.industry Histology General Medicine medicine.disease Immunohistochemistry Mice Inbred C57BL P-Selectin embryonic structures Pediatrics Perinatology and Child Health Models Animal Gestation Wounds and Injuries Surgery medicine.symptom business |
| Zdroj: | Journal of pediatric surgery. 43(4) |
| ISSN: | 1531-5037 |
| Popis: | During early gestation, fetal wounds heal with paucity of inflammation and absent scar formation. P-selectin is an adhesion molecule that is important for leukocyte recruitment to injury sites. We used a murine fetal wound healing model to study the specific contribution of P-selectin to scarless wound repair.Linear excisional wounds were created on the dorsa of E15.5 and E17.5 gestation fetuses in wild-type and P-selectin (-/-) mice (term = 19 days). Wounds were harvested at various time-points after wounding and analyzed using histology and immunohistochemistry.The E15.5 wounds in both wild-type and P-selectin (-/-) fetuses healed scarlessly and with minimal inflammation, whereas E17.5 wounds healed with fibrosis and inflammation. However, the scars of the P-selectin (-/-) wounds appeared slightly different than wild-type. There were significantly more inflammatory cells in E17.5 wild-type wounds 6 hours after injury (P.001), but the difference was no longer significant by 24 hours. Finally, reepithelialization was slower in the E15.5 knockout wounds compared to their wild-type counterparts.Absence of P-selectin delays inflammatory cell recruitment and reepithelialization of fetal wounds; however, scar formation still occurs in late gestation wounds. The contribution of specific molecules to fetal wound healing can be elucidated using murine knockout or transgenic models. |
| Databáze: | OpenAIRE |
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