Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes
| Autor: | Lianhui Wang, Bingbing Gu, Peng Su, Yunfei Liu, Jia Chen, Biqing Bao, Yanrui Pan, Li Tong, Yu Xu |
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| Rok vydání: | 2018 |
| Předmět: |
Biosensing Techniques
02 engineering and technology Conjugated system 010402 general chemistry 01 natural sciences Biochemistry Analytical Chemistry Limit of Detection Fluorescence Resonance Energy Transfer Electrochemistry Environmental Chemistry Spectroscopy Exonuclease III Detection limit biology Chemistry DNA Nucleic acid amplification technique 021001 nanoscience & nanotechnology Polyelectrolytes Conjugated Polyelectrolytes 0104 chemical sciences Exodeoxyribonucleases Förster resonance energy transfer Nucleic acid biology.protein Biophysics 0210 nano-technology Nucleic Acid Amplification Techniques Biosensor |
| Zdroj: | The Analyst. 143:4267-4272 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c8an01024b |
| Popis: | In this paper, a novel ratiometric and cascade amplification strategy was developed by combining the unique signal amplification and effective fluorescence resonance energy transfer (FRET) property of conjugated polymers with the Exo III-assisted target recycling method. The target DNA (ssDNAc) could be hybridized with the duplex-stranded probe to trigger the cyclic digestion of the probe strands and lead to the continuous release of fluorescein from the probe. The proposed strategy thus shows enhanced sensitivity toward target DNA with a detection limit of 0.38 nM, which is more sensitive than the previously reported comparable biosensors based on conjugated polyelectrolytes. Furthermore, this method exhibited an improved performance to discriminate single mismatched targets through an efficient FRET-based ratiometric detection method using a conjugated polymer as a donor and an optical transducer. More importantly, this cascade amplification approach offers the advantages of simplicity, which avoids multiple utilization of probes and complex assay steps required in traditional amplification methods. |
| Databáze: | OpenAIRE |
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