Photoperiodic variations of the polysialylated form of neural cell adhesion molecule within the hypothalamus and related reproductive output in the ewe
| Autor: | Juliette Cognie, Benoît Malpaux, Stephanie Chalivoix, Daniel Guillaume, Laurence Dufourny, Jean-Claude Thiéry |
|---|---|
| Přispěvatelé: | Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Region Centre, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
endocrine system [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] Histology media_common.quotation_subject Photoperiod Hypothalamus Neural Cell Adhesion Molecule L1 Biology Pathology and Forensic Medicine Melatonin 03 medical and health sciences Random Allocation 0302 clinical medicine Internal medicine Neuroplasticity medicine Animals Secretion Rats Wistar 030304 developmental biology media_common seasonal reproduction photoperiodism 0303 health sciences western blot endoneuraminidase n Sheep Reproduction Cell Biology Rats Preoptic area Endocrinology nervous system plasticity polysialylated form of neural cell adhesion molecule Sialic Acids Neural cell adhesion molecule Female 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Cell and Tissue Research Cell and Tissue Research, Springer Verlag, 2013, 352 (2), pp.387-399. ⟨10.1007/s00441-012-1544-z⟩ |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-012-1544-z⟩ |
| Popis: | Remerciements : INRA, UMR PRC, Plate-Forme CIRE, 37380 Nouzilly; Cellular mechanisms induced by melatonin to synchronise seasonal reproduction in several species, including sheep, remain unclear. We sought to evaluate the scale and physiological significance of neural plasticity in order to explain the delay between the change of duration of melatonin secretion and the change of reproductive status following a transition from long days (LD, 16 h light/24 h) to short days (SD, 8 h light/24 h) and from SD to LD. Using Western blots in ovariectomised oestradiol-replaced ewes, we evaluated the content of the polysialylated form of neural cell adhesion molecule (PSA-NCAM), a plasticity marker, in the hypothalamus. From day 15 following a transition to SD, most hypothalamic areas showed a decrease of PSA-NCAM level that was particularly significant in the preoptic area (POA). Following a transition to LD, PSA-NCAM content increased at day 15 in most regions except in the premammillary hypothalamic area (PMH) in which a significant decrease was noted. The functional importance of PSA-NCAM variations for seasonal reproduction was assessed for the PMH and POA. PSA-NCAM was degraded by stereotaxic injections of endoneuraminidase N and luteinising hormone (LH) secretion was recorded in treated and control ewes. Degradation of PSA-NCAM in the PMH in SD-treated ewes failed to produce a significant effect on LH secretion, whereas a similar treatment in the POA before a transition to SD delayed activation of the gonadotroph axis in two-thirds of the ewes. Our results suggest that the photoperiod controls variations of the hypothalamic content of a plasticity marker and that these might be important for the regulation of seasonal reproduction, particularly in the POA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink