Biotechnological Production of Xylitol: Enhancement of Monosaccharide Production by Post-Hydrolysis of Dilute Acid Sugarcane Hydrolysate
| Autor: | Silvio Silvério da Silva, Boutros Fouad Sarrouh, Ricardo de Freitas Branco |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_classification
Chemistry Hydrolysis Monosaccharides food and beverages Bioengineering General Medicine Xylose Xylitol Applied Microbiology and Biotechnology Biochemistry Xylan Hydrolysate Saccharum carbohydrates (lipids) chemistry.chemical_compound Monosaccharide Hemicellulose Fermentation Food science Molecular Biology Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 153:163-170 |
| ISSN: | 1559-0291 0273-2289 |
| Popis: | Dilute-acid hydrolysis pretreatment of sugarcane bagasse resulted in release of 48% (18.4 g/L) of the xylan in the hemicellulose fraction into the hydrolysate as monomeric xylose. In order to enhance the recuperation of this monomer, a post-hydrolysis stage consisted of thermal treatment was carried out. This treatment resulted in an increase in xylose release of 62% (23.5 g/L) of the hemicellulose fraction. Original and post-hydrolysates were concentrated to the same levels of monomeric xylose in the fermentor feed. During the fermentation process, cellular growth was observed to be higher in the post-hydrolysate (3.5 g/L, Y(x/s) = 0.075 g cells/g xylose) than in the original hydrolysate (2.9 g/L, Y(x/s) = 0.068 g cells/g xylose). The post-treated hydrolysate required less concentration of sugars resulting in a lower concentration of fermentation inhibitors, which were formed primarily in the dilute acid hydrolysis step. Post-hydrolysis step led to a high xylose-xylitol conversion efficiency of 76% (0.7 g xylitol/g xylose) and volumetric productivity of 0.68 g xylitol/L h when compared to 71% (0.65 g xylitol/g xylose and productivity of 0.61 g xylitol/L h) for the original hemicellulosic hydrolysate. |
| Databáze: | OpenAIRE |
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