Description of AtCAX4 in Response to Abiotic Stress in Arabidopsis
| Autor: | Shenkui Liu, Weichao Fu, Yuanyuan Bu, Jiangpo Chen, Tetsuo Takano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine abiotic stress Antiporter Arabidopsis interaction Vacuole 01 natural sciences Catalysis Article Antiporters lcsh:Chemistry Inorganic Chemistry Ca2+/H+ antiporters (CAXs) 03 medical and health sciences Bimolecular fluorescence complementation Stress Physiological Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Cation Transport Proteins Spectroscopy Binding Sites biology Chemistry Abiotic stress Arabidopsis Proteins Organic Chemistry Transporter General Medicine Plant cell biology.organism_classification Computer Science Applications Cell biology 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 Mutation Function (biology) 010606 plant biology & botany Protein Binding |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 856, p 856 (2021) Volume 22 Issue 2 |
| ISSN: | 1422-0067 |
| Popis: | High-capacity tonoplast cation/H+ antiport in plants is partially mediated by a family of CAX transporters. Previous studies have reported that CAX activity is affected by an N-terminal autoinhibitory region. CAXs may be present as heterodimers in plant cells, and this phenomenon necessitates further study. In this study, we demonstrate that there is an interaction between CAX4 and CAX1 as determined by the use of a yeast two-hybrid system and a bimolecular fluorescence complementation assay. More specifically, the N-terminal of CAX4 interacts with CAX1. We further observed the over-expression and either a single or double mutant of CAX1 and CAX4 in response to abiotic stress in Arabidopsis. These results suggest that CAX1 and CAX4 can interact to form a heterodimer, and the N-terminal regions of CAX4 play important roles in vivo this may provide a foundation for a deep study of CAX4 function in the future. |
| Databáze: | OpenAIRE |
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