Long Non-Coding RNA LOC648987 Promotes Proliferation and Metastasis of Renal Cell Carcinoma by Regulating Epithelial-Mesenchymal Transition

Autor: Wei Liu, Jian-Jun Lu, Liang Zhou, Qin Yu, Yao-Wu Su
Rok vydání: 2021
Předmět:
Male
Cancer Research
Carcinogenesis
Cell
Vimentin
Apoptosis
medicine.disease_cause
urologic and male genital diseases
Kidney
Metastasis
Mice
0302 clinical medicine
Cell Movement
Neoplasm Metastasis
RNA
Small Interfering
RC254-282
0303 health sciences
biology
EMT
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Cell cycle
Middle Aged
female genital diseases and pregnancy complications
Kidney Neoplasms
medicine.anatomical_structure
Oncology
Matrix Metalloproteinase 9
030220 oncology & carcinogenesis
Gene Knockdown Techniques
Female
Original Article
renal cell carcinoma
Epithelial-Mesenchymal Transition
proliferation
03 medical and health sciences
Cell Line
Tumor
medicine
Animals
Humans
metastasis
Neoplasm Invasiveness
Epithelial–mesenchymal transition
Carcinoma
Renal Cell
030304 developmental biology
Aged
Cell Proliferation
Cell growth
Cell Cycle Checkpoints
medicine.disease
Cell culture
biology.protein
Cancer research
lncRNA LOC648987
Neoplasm Transplantation
Zdroj: Technology in Cancer Research & Treatment
Technology in Cancer Research & Treatment, Vol 20 (2021)
ISSN: 1533-0338
Popis: Renal cell carcinoma (RCC) is a type of urinary tumor with a high incidence and is often associated with tumor metastasis. Long non-coding RNA (lncRNA) regulates tumorigenesis, progression, and metastasis. However, the role and the predictive value of lncRNA in RCC progression and metastasis have not been elucidated. The purpose of this study was to evaluate the effect of a newly discovered lncRNA LOC648987 on RCC proliferation and metastasis. LOC648987 was identified by RT-PCR for high expression in human RCC tissues as well as in metastatic RCC tissues. In the cell experiments, we infected the RCC cell lines ACHN and 786-O cells with LOC648987-shRNA and its negative control (shNC). The results showed that the knockdown of LOC648987 inhibited the proliferation of ACHN and 786-O cells and colony formation. The cell cycle and the apoptosis progression of ACHN and 786-O cells were assessed using flow cytometry. The knockdown of LOC648987 significantly inhibited the progression of ACHN and 786-O cells from G0/G1 to S phase and promoted cell apoptosis. The metastasis promoting effects of LOC648987 on ACHN and 786-O cells were verified by transwell migration assays, which depended on vimentin and MMP-9 to regulate the epithelial–mesenchymal transition. Finally, the promotion of LOC648987 on RCC tumorigenesis was evaluated in BALb/c nude mice. These data confirmed that lncRNA LOC648987 promoted RCC cell proliferation and tumor metastasis and regulated the expression of EMT-related proteins in RCC cells.
Databáze: OpenAIRE
Externí odkaz: