Determination of mycotoxins in beer by multi heart-cutting two-dimensional liquid chromatography tandem mass spectrometry method
| Autor: | Massimo Labra, Serena Rizzo, Mariateresa Russo, Luca Campone, Anna Lisa Piccinelli, Luca Rastrelli, Imma Pagano, Rita Celano |
|---|---|
| Přispěvatelé: | Campone, L, Rizzo, S, Piccinelli, A, Celano, R, Pagano, I, Russo, M, Labra, M, Rastrelli, L |
| Rok vydání: | 2020 |
| Předmět: |
Ochratoxin A
Aflatoxin Analyte Aflatoxin B1 2D chromatography Beers Mycotoxins analysis Food safety LC × LC MS/MS Multi heart-cutting Beers Electrospray ionization Food Contamination Fumonisins 01 natural sciences High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Tandem Mass Spectrometry Liquid chromatography–mass spectrometry Chromatography High Pressure Liquid Mycotoxin Chromatography Chemistry Elution Beers Mycotoxins analysi 010401 analytical chemistry Fumonisin Mycotoxins analysis Beer 04 agricultural and veterinary sciences General Medicine Mycotoxins Ochratoxins 040401 food science 0104 chemical sciences Ochratoxin Two-dimensional chromatography Food Science |
| Zdroj: | Food Chemistry. 318:126496 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2020.126496 |
| Popis: | Beer is one the most consumed alcoholic beverage in the world and its contamination with mycotoxins is of public health concern. This study reports a fast and automated analytical procedure based on a multi-heart-cutting two-dimensional liquid chromatography tandem mass spectrometry method using electrospray ionization for the determination of seven mycotoxins (aflatoxins B1, B2, G2 and G1, ochratoxin A, fumonisins B1 and B2) in beers. The developed method was based on the heart-cutting 2D- HPLC technique in which only the specific portions of the first dimension, in the retention time of analytes, were transferred into the second dimension for the further separation and successive determination. The method uses two different chromatographic columns; in the first dimension, 50 μL of sample was injected on first column, and mycotoxins elution regions were collected in a loop and transferred into the second column for the separation of analytes. Each column operated in gradient elution mode in order to eliminate interfering compounds and improve separation and peak shape. After the optimization, the method has been validated according to EU regulation and finally applied for the analysis of forty beer samples collected from Italian supermarkets. Among all mycotoxins studied, fumonisins B1 was the most widely distributed in analysed beers (>21%) in the range from 0.6 to 12.3 ng mL−1. The automated methodology developed was able to determine accurately and simultaneously seven mycotoxins in beer. This provided a significant reduction of sample handle and, consequently of analysis time. |
| Databáze: | OpenAIRE |
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