Zinc Oxide Nanoparticles Induce Toxicity in H9c2 Rat Cardiomyoblasts

Autor:	Criselda Mendoza-Milla, Fernanda Isabel Macías Macías, Kimberly Abigail Velázquez Delgado, Manuel Alejandro Herrera Rodríguez, Zaira Colín-Val, María del Pilar Ramos-Godinez, Agustina Cano-Martínez, Anita Vega-Miranda, Diana Xochiquetzal Robledo-Cadena, Norma Laura Delgado-Buenrostro, Yolanda Irasema Chirino, José Ocotlán Flores-Flores, Rebeca López-Marure
Rok vydání:	2022
Předmět:	Ions Organic Chemistry Metal Nanoparticles General Medicine zinc oxide nanoparticles toxicity cell death cardiomyoblasts Catalysis Computer Science Applications Rats Inorganic Chemistry Microscopy Electron Transmission Animals Nanoparticles Physical and Theoretical Chemistry Zinc Oxide Molecular Biology Oxidation-Reduction Spectroscopy
Zdroj:	International Journal of Molecular Sciences; Volume 23; Issue 21; Pages: 12940
ISSN:	1422-0067
Popis:	Zinc oxide nanoparticles (ZnO NPs) are widely used in the cosmetic industry. They are nano-optical and nano-electrical devices, and their antimicrobial properties are applied in food packaging and medicine. ZnO NPs penetrate the body through inhalation, oral, and dermal exposure and spread through circulation to various systems and organs. Since the cardiovascular system is one of the most vulnerable systems, in this work, we studied ZnO NPs toxicity in H9c2 rat cardiomyoblasts. Cardiac cells were exposed to different concentrations of ZnO NPs, and then the morphology, proliferation, viability, mitochondrial membrane potential (ΔΨm), redox state, and protein expression were measured. Transmission electron microscopy (TEM) and hematoxylin–eosin (HE) staining showed strong morphological damage. ZnO NPs were not observed inside cells, suggesting that Zn2+ ions were internalized, causing the damage. ZnO NPs strongly inhibited cell proliferation and MTT reduction at 10 and 20 μg/cm2 after 72 h of treatment. ZnO NPs at 20 μg/cm2 elevated DCF fluorescence, indicating alterations in the cellular redox state associated with changes in ΔΨm and cell death. ZnO NPs also reduced the intracellular expression of troponin I and atrial natriuretic peptide. ZnO NPs are toxic for cardiac cells; therefore, consumption of products containing them could cause heart damage and the development of cardiovascular diseases.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e01200d8d7580c1dfb9794340e0a351e https://pubmed.ncbi.nlm.nih.gov/36361726 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.