Activation of murine oocytes with Ca2+ ionophore and cycloheximide
| Autor: | M. L. Leibfried Rutledge, N. L. First, L. J. Hagemann, F. L. Hillery-Weinhold |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Mice Inbred ICR Pronucleus Parthenogenesis Ionophore General Medicine Cycloheximide Biology Male pronucleus Molecular biology In vitro Mice Inbred C57BL Mice chemistry.chemical_compound chemistry Mice Inbred DBA Ca2 ionophore Oocytes Protein biosynthesis Animals Female Animal Science and Zoology Calcimycin |
| Zdroj: | Journal of Experimental Zoology. 271:57-61 |
| ISSN: | 1097-010X 0022-104X |
| Popis: | A Ca2+ ionophore (A23187, 3 microM) and inhibitor of protein synthesis (cycloheximide, 10 micrograms/ml) were used sequentially as a unique method for activating mouse oocytes in vitro. Brief exposure of oocytes to A23187 followed by 6 hr in cycloheximide resulted in a higher activation rate (93.8%) compared to A23187 or cycloheximide alone (37.7% and 36.5%, respectively) or the two reagents in reverse order (29.8%). The parthenogenones consistently contained a single pronucleus and second polar body, and showed a high degree of developmental potential, as assessed by transfer to recipient females or addition of a male pronucleus followed by transfer to recipients. This method is a useful way of obtaining large numbers of activated haploid mammalian oocytes for further developmental studies. |
| Databáze: | OpenAIRE |
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