The Rac activator Tiam1 controls tight junction biogenesis in keratinocytes through binding to and activation of the Par polarity complex
| Autor: | Alexander E.E. Mertens, Rob A. van der Kammen, Cristina Olivo, John G. Collard, Tomasz P. Rygiel |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Keratinocytes
Tight junction Cell Polarity Proteins Cell Biology CDC42 GTPase Biology Tight Junctions rac GTP-Binding Proteins Cell biology Rac GTP-Binding Proteins Mice Cdc42 GTP-Binding Protein Report Cell polarity Animals Guanine Nucleotide Exchange Factors Receptors Thrombin T-Lymphoma Invasion and Metastasis-inducing Protein 1 Guanine nucleotide exchange factor Research Articles Protein Kinase C Biogenesis Signal Transduction |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.200502129 |
| Popis: | The GTPases Rac and Cdc42 play a pivotal role in the establishment of cell polarity by stimulating biogenesis of tight junctions (TJs). In this study, we show that the Rac-specific guanine nucleotide exchange factor Tiam1 (T-lymphoma invasion and metastasis) controls the cell polarity of epidermal keratinocytes. Similar to wild-type (WT) keratinocytes, Tiam1-deficient cells establish primordial E-cadherin–based adhesions, but subsequent junction maturation and membrane sealing are severely impaired. Tiam1 and V12Rac1 can rescue the TJ maturation defect in Tiam1-deficient cells, indicating that this defect is the result of impaired Tiam1–Rac signaling. Tiam1 interacts with Par3 and aPKCζ, which are two components of the conserved Par3–Par6–aPKC polarity complex, and triggers biogenesis of the TJ through the activation of Rac and aPKCζ, which is independent of Cdc42. Rac is activated upon the formation of primordial adhesions (PAs) in WT but not in Tiam1-deficient cells. Our data indicate that Tiam1-mediated activation of Rac in PAs controls TJ biogenesis and polarity in epithelial cells by association with and activation of the Par3–Par6–aPKC polarity complex. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|