Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation

Autor: Dimitra Markala, Panagiota Anyfanti, Zoi Bousiou, Ioanna Sakellari, Ioannis Batsis, Eugenia Gkaliagkousi, Efthalia Yiannaki, Marianna Masmanidou, Achilles Anagnostopoulos, Eleni Gavriilaki, Anna Vardi, Ippokratis Zarifis, Stella Douma, Barbara Nikolaidou, Antonios Lazaridis
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Oncology
Male
Graft vs Host Disease
Disease
030204 cardiovascular system & hematology
lcsh:Chemistry
0302 clinical medicine
Cancer Survivors
Cell-Derived Microparticles
Platelet
Endothelial dysfunction
lcsh:QH301-705.5
Spectroscopy
vascular injury
Hematopoietic Stem Cell Transplantation
General Medicine
Middle Aged
Pathophysiology
Blood Coagulation Factors
humanities
Computer Science Applications
Cardiovascular Diseases
Hematologic Neoplasms
Female
microvesicles
Adult
medicine.medical_specialty
Thrombotic microangiopathy
Adolescent
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Young Adult
Internal medicine
medicine
Humans
Transplantation
Homologous
Physical and Theoretical Chemistry
Molecular Biology
allogeneic hematopoietic cell transplantation
Hematopoietic cell
business.industry
Organic Chemistry
medicine.disease
Microvesicles
Transplantation
pro-coagulant activity
lcsh:Biology (General)
lcsh:QD1-999
Heart Disease Risk Factors
Case-Control Studies
Endothelium
Vascular
business
030215 immunology
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 24
International Journal of Molecular Sciences, Vol 21, Iss 9768, p 9768 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21249768
Popis: (1) Background: survivors of allogeneic hematopoietic cell transplantation (alloHCT) suffer from morbidity and mortality due to cardiovascular events. We hypothesized that vascular injury and pro-coagulant activity are evident in alloHCT survivors without existing alloHCT complications or relapse. (2) Methods: we enrolled consecutive adult alloHCT survivors without established cardiovascular disease and control individuals matched for traditional cardiovascular risk factors (January&ndash
December 2019). Circulating microvesicles (MVs) of different cellular origins (platelet, erythrocyte, and endothelial) were measured by a standardized flow cytometry protocol as novel markers of vascular injury and pro-coagulant activity. (3) Results: we recruited 45 survivors after a median of 2.3 (range 1.1&ndash
13.2) years from alloHCT, and 45 controls. The majority of patients suffered from acute (44%) and/or chronic (66%) graft-versus-host disease (GVHD). Although the two groups were matched for traditional cardiovascular risk factors, alloHCT survivors showed significantly increased platelet and erythrocyte MVs compared to controls. Within alloHCT survivors, erythrocyte MVs were significantly increased in patients with a previous history of thrombotic microangiopathy. Interestingly, endothelial MVs were significantly increased only in alloHCT recipients of a myeloablative conditioning. Furthermore, MVs of different origins showed a positive association with each other. (4) Conclusions: endothelial dysfunction and increased thrombotic risk are evident in alloHCT recipients long after alloHCT, independently of traditional cardiovascular risk factors. An apparent synergism of these pathophysiological processes may be strongly involved in the subsequent establishment of cardiovascular disease.
Databáze: OpenAIRE
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