Effects of Methanol and Temperature on Enzyme Immunoassay with Monoclonal Antibodies Specific to the Insecticide Etofenprox
| Autor: | Kennichi Miyake, Tomoko Kadoya, Hideo Ohkawa, Masanao Katagiri, Fumihide Ishibashi |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Insecticides
Anticorps monoclonal medicine.drug_class Monoclonal antibody Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Antigen-Antibody Reactions Immunoenzyme Techniques chemistry.chemical_compound Antibody Specificity Etofenprox Pyrethrins medicine Molecular Biology chemistry.chemical_classification Chromatography biology medicine.diagnostic_test Methanol Organic solvent Organic Chemistry Temperature Antibodies Monoclonal General Medicine Enzyme chemistry Immunoassay biology.protein Thermodynamics Indicators and Reagents Antibody Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 63:1988-1990 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.63.1988 |
| Popis: | We examined the effects of methanol and temperature on the reactivity of monoclonal antibodies specific to the insecticide etofenprox. When the antigen-antibody reaction was done at 4 degrees C in 10% methanol, the sensitivity in the enzyme immunoassay with each antibody was more than 10-fold higher than that measured at 37 degrees C. Although in 10% methanol one of the antibodies reacted equally with both etofenprox and the carbonate-derivative of etofenprox, in 50% methanol the antibody reacted with etofenprox, but not with the derivative. |
| Databáze: | OpenAIRE |
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