Survival of hippocampal neurons in culture upon hypoxia: Effect of erythropoietin
| Autor: | Piotr Lewczuk, Hannelore Ehrenreich, Martin Hasselblatt, Andrea Heyer, Anna-Leena Sirén, Heike Kamrowski-Kruck, Christian Unzicker |
|---|---|
| Předmět: |
medicine.medical_specialty
Programmed cell death Transcription Genetic Cell Survival Cycloheximide Hippocampal formation Biology Hippocampus chemistry.chemical_compound Internal medicine Receptors Erythropoietin medicine Animals Rats Wistar Receptor Erythropoietin Cells Cultured Neurons Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Hypoxia (medical) Cell Hypoxia Recombinant Proteins Rats Erythropoietin receptor Endocrinology Animals Newborn Gene Expression Regulation chemistry Cell culture Immunology Fibroblast Growth Factor 2 medicine.symptom medicine.drug |
| Zdroj: | Scopus-Elsevier |
| Popis: | The potential of erythropoietin (EPO) to reduce hypoxia-induced cell death has been investigated in 5-day-old primary cultures of rat postnatal hippocampal neurons. Application of EPO (100 pM) at the start of hypoxia resulted in a significant reduction of neuronal death (33.0 +/- 7.5% in cells incubated with EPO vs 56.75 +/- 7.3% in non-treated cells; n = 4, p < 0.021). Similiar results were obtained upon application of cycloheximide (CHX; 1 microM) simultaneously with hypoxia (34.75 +/- 5.6% vs 56.75 +/- 7.3% with and without CHX, respectively, n = 4, p < 0.035), indicating that hypoxia-induced neuronal death is an active, protein synthesis-dependent process. Both, EPO and EPO receptor (EPOR) were found to be expressed after hypoxia in hippocampal neurons in vitro and in vivo. These results demonstrate for the first time that EPO can reverse hypoxia-induced neuronal death when applied simultaneously with the hypoxic stimulus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|