A Fluorescent Base Analog for Probing Triple Helix Formation
| Autor: | Jean-Jacques Toulmé, Frédéric Godde, Serge Moreau, Karine Aupeix |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Pharmacology
Base Sequence DNA Footprinting DNA footprinting DNA Base analog Nucleic Acid Denaturation Fluorescence Thymine chemistry.chemical_compound Crystallography Spectrometry Fluorescence chemistry Biochemistry Duplex (building) Molecular Probes Quinazolines Genetics Nucleic Acid Conformation Spectrophotometry Ultraviolet Fluorescent Dyes Triple helix |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1087-2906 |
| Popis: | Benzo[g]quinazoline-2,4-(1H,3H)-dione (BgQ), a fluorescent thymine analog, was incorporated into an oligopyrimidine (III) able to give rise to a triple-stranded structure by clamping a purine 11-mer (I). The formation of the I-III complex resulted in both a shift of the fluorescence emission maximum and a decreased fluorescence intensity. No such variations were observed on the formation of a Watson-Crick duplex between I and the complementary strand in which a T residue was substituted for BgQ. Therefore, the fluorescence emission of BgQ can be used to selectively monitor the formation of triple helices. |
| Databáze: | OpenAIRE |
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