Functionalized nanogel for treating activated astrocytes in spinal cord injury
| Autor: | Giada Cremonesi, Alessandro Mariani, Xhuljana Mingaj, Arianna Rossetti, Massimiliano De Paola, Filippo Rossi, Pietro Veglianese, Simonetta Papa, Alessandro Sacchetti, Gianluigi Forloni, Emanuele Mauri, Valeria Veneruso |
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| Rok vydání: | 2020 |
| Předmět: |
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Endocytic cycle Pharmaceutical Science Nanogels Inflammation 02 engineering and technology Polyethylene Glycols 03 medical and health sciences In vivo medicine Humans Polyethyleneimine Internalization Spinal cord injury Spinal Cord Injuries 030304 developmental biology media_common 0303 health sciences Chemistry 021001 nanoscience & nanotechnology medicine.disease In vitro Astrogliosis Cell biology Spinal Cord Astrocytes medicine.symptom 0210 nano-technology Nanogel |
| Zdroj: | Journal of controlled release : official journal of the Controlled Release Society. 330 |
| ISSN: | 1873-4995 |
| Popis: | Astrogliosis has a unique reaction during spinal cord damage, with helpful or adverse impacts on recovery. There is consequently a pressing need for treatment to target activated astrocytes and their unsafe response after injury to ensure some preservative effect during the progressive damage. We specifically developed and characterized a functionalized nanogel-based nanovector in vitro and in vivo, demonstrating its selectivity towards astrocytes, and limited uptake by macrophages when functionalized with both NH2 and Cy5 groups. In vitro experiments showed that the internalization was mediated by a clathrin-dependent endocytic pathway. After internalization into the cytoplasm of astrocytes, nanogels undergo lysosomal degradation and release compounds with potential therapeutic efficacy. |
| Databáze: | OpenAIRE |
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