LncRNA SNHG10 is downregulated in non-small cell lung cancer and predicts poor survival
| Autor: | Feng Wu, Shimao Song, Chuanhua Cao, Meng Liang, Linlin Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pulmonary and Respiratory Medicine
Lung Neoplasms Down-Regulation NSCLC Methylation 03 medical and health sciences 0302 clinical medicine Carcinoma Non-Small-Cell Lung Tumor Cells Cultured Medicine Humans Lung cancer 030304 developmental biology Cell Proliferation lcsh:RC705-779 0303 health sciences Cell growth business.industry SNHG10 Nsclc cell lcsh:Diseases of the respiratory system medicine.disease respiratory tract diseases MicroRNAs 030220 oncology & carcinogenesis DNA methylation Cancer research Linear Models RNA Long Noncoding Non small cell miR-21 business Liver cancer Research Article |
| Zdroj: | BMC Pulmonary Medicine, Vol 20, Iss 1, Pp 1-6 (2020) BMC Pulmonary Medicine |
| ISSN: | 1471-2466 |
| DOI: | 10.1186/s12890-020-01281-w |
| Popis: | Background LncRNA SNHG10 has been reported to be an oncogenic lncRNA in liver cancer. However, its roles in non-small cell lung cancer (NSCLC) remains unknown. Methods Tumor and paired non-tumor tissues were harvested from 62 NSCLC patients. RT-qPCR was used to detect the expression of SNHG10 and miR-21 in tissues. Overexpression experiments were used to evaluate the interaction between SNHG10 and miR-21 in NSCLC cells. CCK-8 assay was used to detect the cell proliferation. Results We observed the expression of SNHG10 was down-regulated in non-small cell lung cancer (NSCLC) compared with that in non-tumor tissues. Moreover, we found that high expression levels of SNHG10 predicted favorable survival of NSCLC patients, and the expression of miR-21 were increased in NSCLC and inversely correlated with SNHG10 expression. In NSCLC cells, overexpression of SNHG10 resulted in increased miR-21 gene methylation and decreased miR-21 expression. Moreover, overexpression of SNHG10 attenuated the enhancing effect of miR-21 overexpression on cell proliferation. Conclusions SNHG10 may involve in NSCLC cell proliferation by regulating the miR-21 gene methylation. |
| Databáze: | OpenAIRE |
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