Protein signatures of seminal plasma from bulls with contrasting frozen-thawed sperm viability
| Autor: | Erdogan Memili, Robin Park, Abdullah Kaya, Fabio P. Gomes, Arlindo A. Moura, Einko Topper, A. G. A. Viana, Carolina Fernández-Costa, John R. Yates |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Proteomics Proteome Seminal Plasma Proteins Cell Survival Population lcsh:Medicine Semen Article Flow cytometry Andrology 03 medical and health sciences 0302 clinical medicine Animal physiology medicine Animals education lcsh:Science Cryopreservation education.field_of_study Multidisciplinary medicine.diagnostic_test Chemistry lcsh:R Fertility Preservation Proteasome complex Sperm Spermatozoa Semen Analysis 030104 developmental biology Fertility Gene Ontology Phenotype Cattle lcsh:Q Gelsolin 030217 neurology & neurosurgery Biomarkers Semen Preservation |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The present study investigated the seminal plasma proteome of Holstein bulls with low (LF; n = 6) and high (HF; n = 8) sperm freezability. The percentage of viable frozen-thawed sperm (%ViableSperm) determined by flow cytometry varied from -2.2 in LF to + 7.8 in HF bulls, as compared to the average %ViableSperm (54.7%) measured in an 860-sire population. Seminal proteins were analyzed by label free mass spectrometry, with the support of statistical and bioinformatics analyses. This approach identified 1,445 proteins, associated with protein folding, cell–cell adhesion, NADH dehydrogenase activity, ATP-binding, proteasome complex, among other processes. There were 338 seminal proteins differentially expressed (p 2 = 0.84 and 0.79; p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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