CENP‐50 is required for papilloma development in the two‐stage skin carcinogenesis model
Autor: | Naoko Kagawa, Eriko Isogai, Haruka Munakata, Megumi Saito, Yuichi Wakabayashi, Tatsuo Fukagawa, Kazuhiro Okumura |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Cancer Research Skin Neoplasms Carcinogenesis 9 10-Dimethyl-1 2-benzanthracene Phthalic Acids malignant conversion DMBA Cell Cycle Proteins macromolecular substances Biology medicine.disease_cause Mice 03 medical and health sciences 0302 clinical medicine In vivo Conditional gene knockout medicine Carcinoma Animals Humans two‐stage skin carcinogenesis mouse models CENP Skin Mice Knockout Papilloma Malignant Conversion Neoplasms Experimental Original Articles General Medicine Cell cycle medicine.disease 030104 developmental biology Oncology 030220 oncology & carcinogenesis Carcinogens Cancer research Original Article |
Zdroj: | Cancer Science |
ISSN: | 1349-7006 1347-9032 |
DOI: | 10.1111/cas.14533 |
Popis: | CENP‐50/U is a component of the CENP‐O complex (CENP‐O/P/Q/R/U) and localizes to the centromere throughout the cell cycle. Aberrant expression of CENP‐50/U has been reported in many types of cancers. However, as Cenp‐50/U‐deficient mice die during early embryogenesis, its functions remain poorly understood in vivo. To investigate the role of Cenp‐50/U in skin carcinogenesis, we generated Cenp‐50/U conditional knockout (K14CreER‐Cenp‐50/U fl/fl) mice and subjected them to the 7,12‐dimethylbenz(a)anthracene (DMBA)/terephthalic acid (TPA) chemical carcinogenesis protocol. As a result, early‐stage papillomas decreased in Cenp‐50/U‐deficient mice. In contrast, Cenp‐50/U‐deficient mice demonstrated almost the same carcinoma incidence as control mice. Furthermore, mRNA expression analysis using DMBA/TPA‐induced papillomas and carcinomas revealed that Cenp‐50/U expression levels in papillomas were significantly higher than in carcinomas. These results suggest that Cenp‐50/U functions mainly in early papilloma development and it has little effect on malignant conversion. CENP‐50 is required for papilloma development, but has no function in malignant conversion. |
Databáze: | OpenAIRE |
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