Development of a simple validated spectrofluorometric method for the assay of midodrine in tablets dosage form; Application to content uniformity testing

Autor:	Pakinaz Y, Khashaba, Mahmoud, Abdelgaleel, Sayed M, Derayea, Dalia M, Nagy
Rok vydání:	2022
Předmět:	Midodrine Spectrometry Fluorescence Biological Assay Indicators and Reagents Instrumentation Spectroscopy Atomic and Molecular Physics and Optics Tablets Analytical Chemistry
Zdroj:	Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 273:121046
ISSN:	1386-1425
Popis:	A new sensitive spectrofluorimetric technique has been established for the estimation of midodrine hydrochloride. This method depends on the condensation reaction of ortho-phthalaldehyde with the primary aliphatic amine of midodrine in the presence of 2-mercapto-ethanol. The pH of the medium was adjusted to 9.0 using 0.1 M borate buffer. The fluorescence of the product was measured at wavelength of 451 nm after excitation at 334 nm. The method was rectilinear over a concentrations range of 0.1 to 1.5 µg mL
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dffd4a00553d01dd4b30e8e6c179c13d https://doi.org/10.1016/j.saa.2022.121046 Zobrazit plný text záznamu Full Text from ScienceDirect