Simple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems
| Autor: | Jeong Yoon Park, Kwanghun Chung, Justin Swaney, Sung-Yon Kim, Daniel Goodwin, Jae Hun Cho, Van J. Wedeen, Young Gyun Park, Matthew P. Frosch, H. Sebastian Seung, Margaret McCue, Austin Hubbert, Taeyun Ku, Sara Vassallo, Heejin Choi, Naveed A. Bakh, Evan Murray |
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| Přispěvatelé: | Institute for Medical Engineering and Science, Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology. Department of Chemical Engineering, Picower Institute for Learning and Memory, Murray, Evan, Cho, Jae Hun, Ku, Taeyun, Swaney, Justin Mark, Kim, Sung-Yon, Choi, Heejin, Park, Young-Gyun, Park, Jeong-Yoon, Hubbert, Austin W., McCue, Margaret Grace, Ling, Sara Lynn, Bakh, Naveed Ali, Chung, Kwanghun |
| Rok vydání: | 2015 |
| Předmět: |
Male
Proteomics Tissue architecture Software_GENERAL ComputingMethodologies_SIMULATIONANDMODELING ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION High dimensional Computational biology Biology Bioinformatics Nerve Fibers Myelinated General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine Animals Humans Profiling (information science) ComputingMethodologies_COMPUTERGRAPHICS 030304 developmental biology 0303 health sciences Tissue Preservation Biochemistry Genetics and Molecular Biology(all) Protein Expression Profiling Molecular Imaging 3. Good health Mice Inbred C57BL ComputingMethodologies_PATTERNRECOGNITION Reducing Agents Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Scalability Female Molecular imaging Algorithms 030217 neurology & neurosurgery |
| Zdroj: | PMC |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2015.11.025 |
| Popis: | Combined measurement of diverse molecular and anatomical traits that span multiple levels remains a major challenge in biology. Here, we introduce a simple method that enables proteomic imaging for scalable, integrated, high-dimensional phenotyping of both animal tissues and human clinical samples. This method, termed SWITCH, uniformly secures tissue architecture, native biomolecules, and antigenicity across an entire system by synchronizing the tissue preservation reaction. The heat- and chemical-resistant nature of the resulting framework permits multiple rounds (>20) of relabeling. We have performed 22 rounds of labeling of a single tissue with precise co-registration of multiple datasets. Furthermore, SWITCH synchronizes labeling reactions to improve probe penetration depth and uniformity of staining. With SWITCH, we performed combinatorial protein expression profiling of the human cortex and also interrogated the geometric structure of the fiber pathways in mouse brains. Such integrated high-dimensional information may accelerate our understanding of biological systems at multiple levels. Simons Foundation. Postdoctoral Fellowship Life Sciences Research Foundation Burroughs Wellcome Fund (Career Award at the Scientific Interface) Searle Scholars Program Michael J. Fox Foundation for Parkinson's Research United States. Defense Advanced Research Projects Agency National Institutes of Health (U.S.) (1-U01-NS090473-01) |
| Databáze: | OpenAIRE |
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