The hemodynamic and metabolic effects of spironolactone treatment in acute kidney injury assessed by hyperpolarized MRI
| Autor: | Lotte Bonde Bertelsen, Christoffer Laustsen, Esben Søvsø Szocska Hansen, Per Mose Nielsen, Haiyun Qi, Jakob Lindhardt, Christian Østergaard Mariager, Won Yong Kim, Rasmus Stilling Tougaard |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Time Factors MINERALOCORTICOID RECEPTOR ANTAGONISM C-13 Ischemia Urology Vascular permeability Spironolactone Kidney 030218 nuclear medicine & medical imaging Capillary Permeability 03 medical and health sciences chemistry.chemical_compound AKI 0302 clinical medicine RENAL ISCHEMIA PERFUSION medicine Animals Radiology Nuclear Medicine and imaging Rats Wistar Spectroscopy Evans Blue Analysis of Variance Renal ischemia business.industry Hemodynamics Acute kidney injury Acute Kidney Injury medicine.disease Magnetic Resonance Imaging OPEN-SOURCE SOFTWARE Perfusion Hyperpolarization medicine.anatomical_structure Renal Metabolism chemistry Renal blood flow ALDOSTERONE Molecular Medicine business 030217 neurology & neurosurgery MRI |
| Zdroj: | Lindhardt, J L, Nielsen, P M, Hansen, E S S, Qi, H, Tougaard, R S, Mariager, C Ø, Bertelsen, L B, Kim, W Y & Laustsen, C 2020, ' The hemodynamic and metabolic effects of spironolactone treatment in acute kidney injury assessed by hyperpolarized MRI ', NMR in Biomedicine, vol. 33, no. 10, e4371 . https://doi.org/10.1002/nbm.4371 |
| ISSN: | 1099-1492 0952-3480 |
| DOI: | 10.1002/nbm.4371 |
| Popis: | Renal ischemia-reperfusion injury (IRI) is one of the most common types of acute kidney injury. Spironolactone has shown promising kidney protective effects in renal IRI in rats. We investigated the hemodynamic and metabolic effects of spironolactone (100 mg/kg) administered immediately after 40 min unilateral kidney ischemia in rats. Hyperpolarized MRI using co-polarized [1-13 C]pyruvate and [13 C,15 N2 ]urea as well as 1 H dynamic contrast-enhanced (DCE) MRI was performed 24 h after induction of ischemia. We found a significant decrease in renal blood flow (RBF) in the ischemic kidney compared with the contralateral one measured using DCE and [13 C,15 N2 ]urea. The RBF measured using [1-13 C]pyruvate and [13 C,15 N2 ]urea was significantly altered by spironolactone. The RBFs in the ischemic kidney compared with the contralateral kidney were decreased similarly as measured using both [13 C,15 N2 ]urea and [1-13 C]pyruvate in the spironolactone-treated group. Spironolactone treatment increased the perfusion-corrected pyruvate metabolism by 54% in both the ischemic and contralateral kidney. Furthermore, we showed a correlation between vascular permeability using a histological Evans blue analysis and the ratio of the volumes of distribution (VoDs), ie VoD-[13 C,15 N2 ]urea/VoD-[1-13 C]pyruvate. This suggests that [13 C,15 N2 ]urea/[1-13 C]pyruvate VoD ratio may be a novel indicator of renal vascular permeability associated with renal damage in rodents. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text