Isolation of a Substantial Proportion of Forest Soil Bacterial Communities Detected via Pyrotag Sequencing
| Autor: | Martin Hartmann, William W. Mohn, Gordon R. Stewart, David VanInsberghe |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Bacteria Ecology biology Sequence analysis Molecular Sequence Data Biodiversity Zoology Sequence Analysis DNA Ribosomal RNA biology.organism_classification Isolation (microbiology) Applied Microbiology and Biotechnology Trees Microbial Ecology Bacterial genetics Pyrosequencing Soil microbiology Soil Microbiology Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 79:2096-2098 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.03112-12 |
| Popis: | We isolated 1,264 bacterial strains from forest soils previously surveyed via pyrosequencing of rRNA gene amplicons. Conventional culturing techniques recovered a substantial proportion of the community, with isolates representing 22% of 98,557 total pyrotags. Growth characteristics of isolates indicated that ecological traits were associated with relative abundances of corresponding pyrotag operational taxonomic units. |
| Databáze: | OpenAIRE |
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