Dissolution of calcium pyrophosphate crystals by polyphosphates: an in vitro and ex vivo study
| Autor: | D Chindamo, Renzo Cini, Enrico Selvi, Roberto Marcolongo, F Nerucci, Maria Pia Picchi, Sauro Lorenzini, Berti G, M Catenaccio |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Metaphosphate medicine.medical_treatment Immunology chemistry.chemical_element Chondrocalcinosis Calcium Calcium Pyrophosphate Statistics Nonparametric General Biochemistry Genetics and Molecular Biology Inhibitory Concentration 50 chemistry.chemical_compound Chondrocytes Rheumatology Polyphosphates Culture Techniques medicine Humans Immunology and Allergy Saline Aged Analysis of Variance business.industry Spectrophotometry Atomic Polyphosphate Middle Aged Phosphate medicine.disease Surgery Extended Report Microscopy Electron Solubility chemistry Female Microscopy Polarization Formazan business Ex vivo Nuclear chemistry |
| Zdroj: | Annals of the Rheumatic Diseases. 60:962-967 |
| ISSN: | 0003-4967 |
| DOI: | 10.1136/ard.60.10.962 |
| Popis: | Objective—To determine the dissolving ability (DA) of linear pentasodium tripolyphosphate (PSTP), cyclic trisodium metaphosphate (TSMP), polymeric sodium metaphosphate (SMP) on synthetic crystals of calcium pyrophosphate dihydrate (CPPD) and on crystalline aggregates of menisci from patients with chondrocalcinosis (CC). Methods—Synthetic CPPD crystals were mixed with phosphate buVered saline (PBS), which contained the diVerent polyphosphates, for one hour at 37°C. The calcified menisci were obtained from the knees of four female patients with CPPD disease who underwent total arthroscopic meniscectomy for degenerative meniscal lesions. Meniscal cryosections and fragments were incubated in SMP (15 mg/ml PBS) at 37°C for one hour and 24 hours, respectively. Histological evaluation on meniscal samples after polyphosphate incubation was carried out by ordinary transmitted light microscopy and polarised light microscopy. The dissolution of CPPD crystals by polyphosphates was assessed by atomic absorption spectroscopy, which determined the amount of calcium liberated from synthetic crystals and meniscal fragments. Cytotoxicity of SMP was evaluated by tetrazolium salt assay and by an ultrastructural study on cultured chondrocytes. Results—SMP and PSTP showed higher DA on CPPD crystals than TSMP. Analysis of the DA values at increasing concentrations of SMP showed that a concentration of 15 mg/ml completely dissolved 2.0 mg CPPD crystals.The solution of meniscal CPPD crystals showed a significant increase of calcium concentration after three hours and 24 hours of SMP incubation (p=0.0001; Kruskal-Wallis analysis of variance) compared with fragments incubated in PBS control solution. Macroscopic and microscopic evaluation of meniscal specimens showed a notable reduction of CPPD deposits. A 50% inhibitory dose on cultured chondrocytes was reached at the maximum concentration of SMP used in this work (15 mg/ml); ultrastructural analysis did not show morphological alterations in the treated cells. Conclusion—The results of this study indicate that linear polyphosphates are eVective in dissolving both synthetic and ex vivo CPPD crystal aggregates. This suggests a potential therapeutic use for these molecules in the treatment of symptomatic CC. (Ann Rheum Dis 2001;60:962‐967) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|