Anti-inflammatory properties of potato glycoalkaloids in stimulated Jurkat and Raw 264.7 mouse macrophages
| Autor: | Mohammad B. Hossain, Catherine M. McCarthy, Anita R. Maguire, Peter W. Jones, Nigel P. Brunton, Nora M. O'Brien, Stuart G. Collins, Olivia Kenny, Dilip K. Rai |
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| Rok vydání: | 2013 |
| Předmět: |
Lipopolysaccharides
medicine.drug_class medicine.medical_treatment Anti-Inflammatory Agents Diosgenin Nitric Oxide Jurkat cells General Biochemistry Genetics and Molecular Biology Anti-inflammatory Cell Line Solanidine Nitric oxide Jurkat Cells Mice chemistry.chemical_compound Concanavalin A medicine Animals Humans General Pharmacology Toxicology and Pharmaceutics Solanum tuberosum biology Plant Extracts Macrophages Interleukin-8 food and beverages General Medicine In vitro Solanine Cytokine chemistry Biochemistry Cell culture biology.protein Interleukin-2 |
| Zdroj: | Life Sciences. 92:775-782 |
| ISSN: | 0024-3205 |
| Popis: | Aims The potato glycoalkaloids, α-chaconine, α-solanine and solanidine, along with potato peel extracts were investigated for potential anti-inflammatory effects in vitro. Their potential to reduce two biomarkers of inflammation, cytokine and nitric oxide (NO) productions, were assessed in the stimulated Jurkat and macrophage models, respectively. Main methods Cytokine and nitric oxide productions were stimulated in Jurkat and Raw 264.7 macrophages with Concanavalin A (Con A; 25 μg/ml) and lipopolysaccaride (LPS; 1 μg/ml), respectively. Selective concentrations of glycoalkaloids and potato peel extracts were added simultaneously with Con A or LPS for 24 h to investigate their potential to reduce inflammatory activity. Key findings α-Chaconine and solanidine significantly reduced interleukin-2 (IL-2) and interleukin-8 (IL-8) productions in Con A-induced Jurkat cells. The potato peel extracts did not influence cytokine production. In LPS-stimulated Raw macrophages, α-solanine, solanidine and two potato peel extracts significantly reduced induced NO production. Significance Our findings suggest that sub-cytotoxic concentrations of potato glycoalkaloids and potato peel extracts possess anti-inflammatory effects in vitro and with further investigation may be useful in the prevention of anti-inflammatory diseases. |
| Databáze: | OpenAIRE |
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