Design of Activatable NIR-II Molecular Probe for In Vivo Elucidation of Disease-Related Viscosity Variations
| Autor: | Songjiao Li, Weijing Huang, Kun Dou, Yunhui Xiang, Zhihong Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Infrared Rays Quantum yield 010402 general chemistry 01 natural sciences Streptozocin Analytical Chemistry Diabetes Mellitus Experimental Viscosity Mice In vivo Microscopy Molecule Animals Fluorescent Dyes Molecular Structure Chemistry Liver Diseases 010401 analytical chemistry Optical Imaging Fluorescence 0104 chemical sciences Microscopy Fluorescence Biophysics Molecular probe Injections Intraperitoneal |
| Zdroj: | Analytical chemistry. 92(6) |
| ISSN: | 1520-6882 1000-1700 |
| Popis: | A clear elucidation of a disease-related viscosity change in vivo is significant yet highly challenging as well. Fluorescence imaging in the second near-infrared region (NIR-II, 1000-1700 nm) has gained increasing attention for observation in living organisms, but a viscosity-activatable fluorescent probe emitting at this region remains a vacancy. Herein, we report the first panel of a viscosity-activated NIR-II emissive fluorescent probe WD-X. By embedding different substituents into the WD-X platform and screening, we obtained an ideal probe, WD-NO2, which displayed the best combination of properties, including a 31-fold fluorescence enhancement in response to viscosity, insensitivity to environments (pH, polarity), and relatively high quantum yield (1.6% in glycerol). WD-NO2 was successfully applied to track the variation of viscosity in diabetes-induced liver injury in vivo. |
| Databáze: | OpenAIRE |
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