Following the stability of amphiphilic nanoaggregates by using intermolecular energy transfer
| Autor: | L. Vander Elst, Hideaki Mizuno, Michael Harris, Tatjana N. Parac-Vogt, Elke Debroye, Yasuhiko Fujita, H. De Keersmaecker |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Stereochemistry
02 engineering and technology 010402 general chemistry 01 natural sciences Micelle Catalysis HeLa Amphiphile Materials Chemistry Fluorescence microscope Rotational correlation time biology Chemistry Metals and Alloys General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Targeted drug delivery Drug delivery Ceramics and Composites Biophysics 0210 nano-technology Fetal bovine serum |
| Popis: | An intermolecular energy transfer system is developed for studying the stability of nanoaggregate(s) (NAs) in complex solution and cell culture by one- and two-photon fluorescence microscopy and optical imaging. The system allows facile addition of one or more tumor targeting molecules, one of which is exemplified here. NAs functionalized with an MRI and optical probe, with and without folic acid, remain stable in fetal bovine serum for at least 4 hours. HeLa cell cultures showed a clear difference between NAs non-targeted and targeted to folate receptors, with both NAs appearing to be taken up by the cells through different mechanisms. An MRI relaxivity, r1, of 9 mM-1 s-1 at 310 K and 1.4 T was measured associated with the increased rotational correlation time of the NAs. These NAs may have application in the targeted drug delivery of hydrophobic drugs such as doxorubicin (DOX). crosscheck: This document is CrossCheck deposited related_data: Supplementary Information copyright_licence: The Royal Society of Chemistry has an exclusive publication licence for this journal copyright_licence: This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0) history: Received 22 September 2016; Accepted 18 October 2016; Accepted Manuscript published 18 October 2016; Advance Article published 27 October 2016; Version of Record published 8 November 2016 ispartof: CHEMICAL COMMUNICATIONS vol:52 issue:91 pages:13385-13388 ispartof: location:England status: published |
| Databáze: | OpenAIRE |
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