Thiamine and riboflavin inhibit production of cytokines and increase the anti-inflammatory activity of a corticosteroid in a chronic model of inflammation induced by complete Freund's adjuvant
| Autor: | Renes R. Machado, Ivo S.F. Melo, Felipe F. Rodrigues, Tony P. Paulino, Márcio M. Coelho, Giovanna M.E. Coura, Caryne M. Bertollo, Adriana M. Godin, Milene Alvarenga Rachid, Raquel R. Menezes, Ana Mercy S. Brito |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.drug_class medicine.medical_treatment Riboflavin Freund's Adjuvant Anti-Inflammatory Agents Inflammation Pharmacology Anti-inflammatory Dexamethasone Rats Sprague-Dawley 03 medical and health sciences medicine Animals Thiamine Chemistry food and beverages General Medicine Rats B vitamins 030104 developmental biology Cytokine Gene Expression Regulation Anesthesia Chronic Disease Corticosteroid Cytokines Drug Therapy Combination Female medicine.symptom human activities medicine.drug |
| Zdroj: | Pharmacological reports : PR. 69(5) |
| ISSN: | 2299-5684 |
| Popis: | Background The effects induced by thiamine and riboflavin, isolated or in association with corticosteroids, in models of chronic inflammation are not known. Thus, we evaluated the effect induced by these B vitamins, isolated or in association with dexamethasone, on the mechanical allodynia, paw edema and cytokine production induced by complete Freund’s adjuvant (CFA) in rats. Methods Chronic inflammation was induced by two injections of CFA. Nociceptive threshold, paw volume and body temperature were evaluated for 21 days. Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) contents were determined in paw tissue. Riboflavin (125, 250 or 500 mg/kg) or thiamine (150, 300 or 600 mg/kg) were administered per os ( po ), twice daily. Dexamethasone (0.5 mg/kg day, po ) was administered every three days. Results CFA induced long lasting mechanical allodynia and paw edema. Elevation of body temperature was observed for a short period. Riboflavin reduced neither paw edema nor mechanical allodynia. Thiamine did not change paw edema, but partially inhibited mechanical allodynia. Riboflavin (500 mg/kg) and thiamine (600 mg/kg) exacerbated the anti-inflammatory activity of dexamethasone. Riboflavin, thiamine and dexamethasone reduced TNF-α and IL-6 production. The association of dexamethasone with thiamine induced greater inhibition of IL-6 production when compared with that induced by dexamethasone. Conclusions Riboflavin and thiamine exacerbate the anti-inflammatory activity of dexamethasone and reduce production of TNF-α and IL-6. |
| Databáze: | OpenAIRE |
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