Anti-Atherosclerotic Properties of Wild Rice in Low-Density Lipoprotein Receptor Knockout Mice: The Gut Microbiome, Cytokines, and Metabolomics Study
Autor: | Andy I Seo, Mohammed H. Moghadasian, Branden S. J. Gregorchuk, Khuong Le, Kayla Kostal, Denice C. Bay, Ramandeep Kaur, Gaia Favero, Gabor Fischer, Mahboubeh Molaei, Vanessa Leung-Shing, Rita Rezzani, Akhila A Joshi, Michelle Wuzinski, Francesca Bonomini |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Male
030309 nutrition & dietetics carbohydrates microbiome Biology Poaceae Article Microbiology functional food 03 medical and health sciences Feces Mice Metabolomics LDL-r-KO mice 16S rDNA Blood plasma Animals Microbiome wild rice Receptor plasma 030304 developmental biology 2. Zero hunger Mice Knockout 0303 health sciences Nutrition and Dietetics Atherosclerosis Carbohydrates Cytokines Functional food Plasma Proteins Wild rice Animal Feed cytokines proteins Diet Gastrointestinal Microbiome Interleukin 10 Gene Expression Regulation Receptors LDL Knockout mouse LDL receptor atherosclerosis Biomarkers Food Science Lipoprotein |
Zdroj: | Nutrients Volume 11 Issue 12 |
ISSN: | 2072-6643 |
Popis: | Background and aim: We previously reported the anti-atherogenic properties of wild rice in low-density lipoprotein receptor knockout (LDL-r-KO) mice. The present study aimed to discover the mechanism of action for such effects. Materials: Fecal and plasma samples from the wild rice treated and control mice were used. Fecal bacterial population was estimated while using 16S rDNA technology. The plasma samples were used to estimate the levels of 35 inflammatory markers and metabolomics, while using Meso Scale multiplex assay and liquid chromatography-mass spectrometry (LC-MS/MS) techniques. Results: Many bacteria, particularly Anaeroplasma sp., Acetatifactor sp., and Prophyromonadaceae sp., were found in higher quantities in the feces of wild rice fed mice as compared to the controls. Cytokine profiles were significantly different between the plasma of treated and control mice. Among them, an increase in the level of IL-10 and erythropoietin (EPO) could explain the anti-atherogenic properties of wild rice. Among many metabolites tested in plasma of these animals, surprisingly, we found an approximately 60% increase in the levels of glucose in the wild rice fed mice as compared to that in the control mice. Conclusion: Additional studies warrant further investigation of the interplay among gut microbiome, inflammatory status, and macronutrient metabolism. |
Databáze: | OpenAIRE |
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