Preparation and characterization of phospholipid-conjugated indocyanine green as a near-infrared probe
| Autor: | Akiko Suganami, Hiroshi Shirasawa, Akira Aoki, Yutaka Tamura, Hisahiro Matsubara, Hideki Hayashi, Hiroshi Kawahira, Tomoyuki Madono, Shigetoshi Okazaki, Yutaka Muraki, Katsuhiko Miyamoto, Kengo Saito, Taro Toyota, Yasunori Akutsu, Takashige Omatsu |
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| Rok vydání: | 2012 |
| Předmět: |
Indocyanine Green
Models Molecular Biodistribution Fluorophore Infrared Rays Lipid Bilayers Clinical Biochemistry Pharmaceutical Science Photochemistry Biochemistry Mice chemistry.chemical_compound Drug Discovery Animals Humans Molecular Biology Fluorescent Dyes Liposome Molecular Structure Chemistry Singlet oxygen Phosphatidylethanolamines Organic Chemistry technology industry and agriculture Neoplasms Experimental Photothermal therapy Fluorescence Heat generation Liposomes Molecular Medicine Indocyanine green |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 22:7481-7485 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/j.bmcl.2012.10.044 |
| Popis: | We have rationally designed and synthesized a novel near-infrared (NIR) photoactivating probe, designated by iDOPE, in which an indocyanine green (ICG) fluorophore is covalently conjugated with a phospholipid moiety, 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine (DOPE), to incorporate into liposome bilayers. NIR irradiation showed that iDOPE retained the optical and fluorescence properties of ICG and demonstrated photoactivator characteristics: fluorescence emission at around 820 nm in a solvent, singlet oxygen production, and concentration-dependent heat generation. Additionally, iDOPE was incorporated into liposome bilayers and maintained stable liposomally formulated iDOPE (LP-iDOPE) over 1 week under physiological conditions. We also observed the tumor-specific biodistribution of LP-iDOPE of in vivo xenografts. These findings suggest that LP-iDOPE might be a promising tool for NIR optical imaging, photodynamic therapy, and photothermal therapy. |
| Databáze: | OpenAIRE |
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