Frequency, clinical characteristics, and genotype distribution of rotavirus gastroenteritis in Greece (2007-2008)

Autor: George A. Syrogiannopoulos, Georgios Trimis, Eleftheria Roma, Heleni Papadopoulou, Antigoni Siamopoulou-Mavridou, Athanassios Michos, Emmanuel Roilides, Emmanuel Galanakis, Elias Iosifidis, Ioanna N. Grivea, Vassiliki Syriopoulou, Dimitra Koukou, Maria Kalmanti, Evagelia Farmaki, Maria Theodoridou, Heleni Tsioni
Přispěvatelé: First Department of Pediatrics, Athens University, Aghia Sophia Children's Hospital, Pediatric Department, University Hospital of Larissa, Department of Pediatrics, University Hospital of Ioannina, University Hospital of Heraklion, First Department of Pediatrics, Aristotle University, Ippokration Hospital of Thessaloniki, Third Department of Pediatrics, Aristotle University
Rok vydání: 2010
Předmět:
Rotavirus
Male
Genotype
Greece/epidemiology
Reoviridae
Rotavirus/*classification/*genetics/isolation & purification
Rotavirus gastroenteritis
medicine.disease_cause
Group A
Rotavirus Infections
Feces
03 medical and health sciences
Virology
Prevalence
Humans
Medicine
Prospective Studies
Rotavirus Infections/*epidemiology/pathology/*virology
Prospective cohort study
Antigens
Viral
Genotyping
030304 developmental biology
0303 health sciences
Greece
Antigens
Viral/analysis
biology
Reverse Transcriptase Polymerase Chain Reaction
030306 microbiology
business.industry
Gastroenteritis/*epidemiology/pathology/*virology
Infant
Sequence Analysis
DNA
Acute gastroenteritis
biology.organism_classification
Gastroenteritis
3. Good health
RNA
Viral/genetics
Infectious Diseases
Child
Preschool
RNA
Viral
Female
business
Feces/virology
Zdroj: Journal of Medical Virology
Journal of Medical Virology, Wiley-Blackwell, 2010, 83 (1), pp.165. ⟨10.1002/jmv.21945⟩
ISSN: 0146-6615
1096-9071
DOI: 10.1002/jmv.21945
Popis: Rotavirus is the leading cause of acute gastroenteritis among young children worldwide. A prospective multi-center study was conducted (2007-2008) in five Pediatric Hospitals to determine the prevalence, the clinical characteristics, and genotype distribution of rotavirus infection in Greece. Faecal samples were examined for the presence of group A rotavirus antigen by immunochromatography. Rotavirus strains were subjected to G and P genotyping by reverse-transcriptase polymerase chain reaction (PCR) and sequencing. A total of 393 children (216 boys) of median age 23 months, participated in the study. Rotavirus was the cause of acute gastroenteritis in 166 children, 42.3% (CI 95%, 37.4-47.1%) of non-hospitalized and 47.8% (CI 95%, 41.7-53.9%) of hospitalized patients. Rotavirus gastroenteritis occurred between December and April in 78.6% of the cases. Most children with RVG (77.8%) were between 3 months and 3 years old. The mean value of Clark severity score was 12.9 +/- 5.1 for RVG and 10.5 +/- 4.9 for non-RVG (P < 0.01). Genotypes were determined in 117 strains and their distribution was as following: G1P[8], 49%; G2P[4], 31%; G4P[8], 10%; G9P[8], 9%; and G8P[14], 1%. In conclusion, rotavirus is a frequent cause of acute gastroenteritis in Greece. The genotypes circulating are similar with those of other European countries. J Med Virol
Databáze: OpenAIRE
Externí odkaz: