Plasma Peptidome Pattern of Breast Cancer Using Magnetic Beads-Based Plasma Fractionation and MALDI-TOF MS: A Case Control Study in Egypt
| Autor: | Hala Ghareeb, Ahmad Elkarmouty, Ahmad Mohamed Zaki, Ragaa A Ramadan, Pacint Moez |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Proteomics
0301 basic medicine Proteome Down-Regulation Breast Neoplasms Chemical Fractionation Sensitivity and Specificity magnetic beads 03 medical and health sciences 0302 clinical medicine Breast cancer Healthy control medicine Humans Blood plasma fractionation Chromatography Chemistry Hydrophilic interaction chromatography Case-control study External validation General Medicine Middle Aged medicine.disease Up-Regulation ClinProTools Matrix-assisted laser desorption/ionization 030104 developmental biology Case-Control Studies Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization 030220 oncology & carcinogenesis Matrix assisted laser desorption ionization/ time of flight mass spectrometry Egypt Female Time-of-flight mass spectrometry Peptides Software Research Article |
| Zdroj: | Asian Pacific Journal of Cancer Prevention : APJCP |
| ISSN: | 2476-762X |
| DOI: | 10.31557/apjcp.2019.20.1.175 |
| Popis: | Objective: The present study aimed to determine peptidome patterns in breast cancer (BC). Methods: We analyzed the plasma proteomic profiling of 80 BC patients and 50 healthy controls, using hydrophobic interaction chromatography magnetic beads (MB-HIC8) separation followed by Matrix assisted laser desorption ionization/ time of flight mass spectrometry (MALDI-TOF MS). Results: ClinProTools software identified 92 peaks that differed among the analyzed groups, 33 peaks were significantly different (P < 0.05). Of those, 22 peaks were up-regulated while 11 peaks were down-regulated in BC patients compared with the healthy controls. Three peptide ion signatures (m/z 1,570.31, 1,897.4 and 2,568.17) were provided by the Quick Classifier model to discriminate BC patients from healthy control subjects with 96.4% accuracy. External validation was performed by an independent group and this achieved a sensitivity of 100% and a specificity of 76.9%. Conclusion: MALDI-TOF MS has good analytical performance in distinguishing BC patients from healthy controls. |
| Databáze: | OpenAIRE |
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