A multiplex immunoassay method for simultaneous quantification of iron, vitamin A and inflammation status markers

Autor: Chris Lyman, Daniel Stevens, David S. Boyle, Carol Levin, Christopher H. Crudder, Dean A. Garrett, Eleanor Brindle
Rok vydání: 2014
Předmět:
Male
Physiology
Epidemiology
lcsh:Medicine
Plant Science
Global Health
chemistry.chemical_compound
Immune Physiology
Medicine and Health Sciences
Multiplex
Public and Occupational Health
lcsh:Science
Vitamin A
Immune Response
Immunoassay
education.field_of_study
Multidisciplinary
biology
medicine.diagnostic_test
Vitamin A Deficiency
Nutritional Deficiencies
Iron deficiency
Iron Deficiencies
Orosomucoid
Vitamins
Micronutrient
Healthy Volunteers
3. Good health
Body Fluids
C-Reactive Protein
Blood
Micronutrient Deficiencies
Epidemiological Methods and Statistics
Female
Anatomy
Research Article
Vitamin
Adult
Iron
Population
Immunology
Disease Surveillance
Receptors
Transferrin
medicine
Humans
education
Soluble transferrin receptor
Nutrition
Inflammation
Biology and life sciences
Population Biology
business.industry
lcsh:R
Malnutrition
Nutrients
Plant Pathology
medicine.disease
Ferritin
Biomarker Epidemiology
chemistry
Ferritins
biology.protein
Iron Deficiency
lcsh:Q
business
Retinol-Binding Proteins
Plasma
Biomarkers
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 12, p e115164 (2014)
ISSN: 1932-6203
Popis: Deficiencies of vitamin A and iron affect a significant portion of the world's population, and efforts to characterize patterns of these deficiencies are hampered by a lack of measurement tools appropriate for large-scale population-based surveys. Vitamin A and iron are not easily measured directly, so reliable proxy markers for deficiency status have been identified and adopted. Measurement of inflammatory markers is necessary to interpret vitamin A and iron status markers, because circulating levels are altered by inflammation. We developed a multiplex immunoassay method for simultaneous measurement of five markers relevant to assessing inflammation, vitamin A and iron status: α-1-acid glycoprotein, C-reactive protein, retinol binding protein 4, ferritin and soluble transferrin receptor. Serum and plasma specimens were used to optimize the assay protocol. To evaluate assay performance, plasma from 72 volunteers was assayed using the multiplex technique and compared to conventional immunoassay methods for each of the five markers. Results of the new and conventional assay methods were highly correlated (Pearson Correlations of 0.606 to 0.991, p
Databáze: OpenAIRE
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