Fyn kinase regulates type II PtdIns 4-kinases in RBL 2H3 cells

Autor: Naveen Bojjireddy, Gosukonda Subrahmanyam, Ranjeet Kumar Sinha
Rok vydání: 2013
Předmět:
Mast-Cell
Phosphatidylinositol 4-phosphate
Clinical Biochemistry
Activation
Fc Epsilon Ri Receptor
Src Family Kinases
Proto-Oncogene Proteins c-fyn
SH2 domain
environment and public health
Tyrosine Kinases
chemistry.chemical_compound
FYN
Cell Line
Tumor
Animals
Humans
Protein Interaction Domains and Motifs
Phosphatidylinositol
Phosphorylation
Beta-Hexosamindase
1-Phosphatidylinositol 4-Kinase
Molecular Biology
Sh2 Domain
Receptors
IgE
Affinity Ige Receptor
Chemistry
Kinase
Fc-Epsilon-Ri
Phosphatidylinositol 4-Phosphate
Rat Splenic Lymphocytes
hemic and immune systems
Tyrosine phosphorylation
Cell Biology
General Medicine
Key Component
beta-N-Acetylhexosaminidases
Rats
Cell biology
Enzyme Activation
enzymes and coenzymes (carbohydrates)
Rbl-2h3 Cells
Phosphatidylinositol 4-Kinase
Protein Processing
Post-Translational
Tyrosine kinase
Protein Binding
Signal Transduction
Zdroj: Molecular and Cellular Biochemistry. 387:63-70
ISSN: 1573-4919
0300-8177
DOI: 10.1007/s11010-013-1871-8
Popis: Type II phosphatidylinositol 4-kinases are implicated in Fc epsilon RI-mediated signaling cascades leading to release of inflammatory molecules. Cross-linking of Fc epsilon RI on RBL 2H3 cells results in protein tyrosine phosphorylation and activation of type II PtdIns 4-kinase activity. Protein tyrosine kinase(s) that phosphorylate type II PtdIns 4-kinase(s) in RBL 2H3 cells remains elusive and is being addressed in this manuscript. Anti-Fyn kinase antibodies co-immunoprecipitated type II PtdIns 4-kinase activity from Fc epsilon RI cross-linked RBL 2H3 cells. In reciprocal assays, His-tagged types II PtdIns 4-kinases were shown to pull down Fyn kinase. Further, anti-Fyn immunoprecipitates were shown to phosphorylate type II PtdIns 4-kinase alpha and beta in in vitro assays. Pull down studies with GST-Fyn-SH2 and GST-Fyn-SH3 domains showed that type II PtdIns 4-kinases associate with Fyn-SH2 domain. Knockdown of Fyn kinase in RBL 2H3 cells abrogated activation of type II PtdIns 4-kinase activity in response to Fc epsilon RI cross-linking and type II PtdIns 4-kinase activity in anti-phosphotyrosine immunoprecipitates. Knockdown of Fyn kinase was also strongly correlated with a reduction in beta-hexosaminidase release in response to Fc epsilon RI cross-linking. These results suggest that type II PtdIns 4-kinases act downstream of Fyn kinase in Fc epsilon RI signaling cascades and are regulated by Fyn kinase.
Databáze: OpenAIRE
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