Distribution of Cryptosporidium species isolated from diarrhoeic calves in Japan
| Autor: | Konosuke Otomaru, Megumi Itoh, Yuto Kano, Fumi Murakoshi, Masayuki Kachi, Ayman Ahmed Shehata, Xuenan Xuan, Mohamed Ibrahim Essa, Yasuhiro Fukuda, Toshie Nakayama, Hironori Bando, Akira Goto, Kei Kazama, Takuto Endo, Kentaro Kato, Atsushi Fujikura, Hiroaki Okawa, Mohammad Hazzaz Bin Kabir, Shoko Oishi |
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| Rok vydání: | 2020 |
| Předmět: |
Diarrhea
Male 0301 basic medicine Veterinary medicine Cryptosporidium infection animal diseases 030231 tropical medicine Cattle Diseases Cryptosporidiosis Cryptosporidium Biology Cryptosporidium species 18S ribosomal RNA 03 medical and health sciences 0302 clinical medicine Japan parasitic diseases Genotype Prevalence RNA Ribosomal 18S medicine Animals Feces DNA Protozoan 030108 mycology & parasitology biology.organism_classification medicine.disease Infectious Diseases Cryptosporidium parvum Cattle Female Parasitology Nested polymerase chain reaction |
| Zdroj: | Parasitology International. 78:102153 |
| ISSN: | 1383-5769 |
| DOI: | 10.1016/j.parint.2020.102153 |
| Popis: | Cryptosporidium spp. are enteric protozoan parasites that infect a wide range of hosts including humans, and domestic and wild animals. The aim of this study was to molecularly characterize the Cryptosporidium spp. found in calf faeces in Japan. A total of 80 pre-weaned beef and dairy calves' diarrhoeic faecal specimens were collected from nine different prefectures in Japan. A nested polymerase chain reaction targeting the small subunit 18S rRNA and GP60 genes were used to detect the Cryptosporidium genotypes and subtypes. 83.8% (67 out of 80) of the specimens were positive for Cryptosporidium spp.; Cryptosporidium was found in both beef and dairy calves. Cryptosporidium parvum was the predominant species, detected in 77.5% (31/40) of beef calves and 80% (32/40) of dairy calves. Cryptosporidium bovis was also detected, 5.0% (2/40) of dairy calves, and C. ryanae was also found 2.5% (1/40) of dairy calves. One mixed-species infection, 2.5% (1/40) was detected in a beef calf having C. parvum, and C. ryanae. We detected the most common subtype of C. parvum (i.e., IIaA15G2R1), as well as other subtypes (i.e., IIaA14G3R1, IIaA14G2R1, and IIaA13G1R1) that have not previously been detected in calves in Japan. Our results demonstrate the widespread diversity of Cryptosporidium infection in calves in Japan. |
| Databáze: | OpenAIRE |
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