Genetic, Morphological and Antigenic Relationships between Mesonivirus Isolates from Australian Mosquitoes and Evidence for Their Horizontal Transmission
Autor: | John Haniotis, Breeanna J. McLean, Christina Hoare, Helle Bielefeldt-Ohmann, Jody Hobson-Peters, Joanne R. Kizu, Roy A. Hall, Sonja Hall-Mendelin, David Warrilow, Natalee D. Newton, Devina Paramitha, Agathe M. G. Colmant, Daniel Watterson, Babak Shaban, Stephen L. Doggett, Cheryl A. Johansen, Andrew F. van den Hurk, Penelope J. Gauci, Caitlin A. O’Brien, Emma L. Ledger, Wen Jun Liu |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
medicine.drug_class insect-specific virus 030231 tropical medicine lcsh:QR1-502 Nidovirales Monoclonal antibody FTA card lcsh:Microbiology Article Virus 03 medical and health sciences 0302 clinical medicine Virology Disease Transmission Infectious medicine Animals Coronaviridae Antigens Viral Phylogeny Genetics biology Australia Virion RNA Sequence Analysis DNA biology.organism_classification Mesoniviridae nidovirus Culicidae 030104 developmental biology Infectious Diseases Vietnam monoclonal antibody Nucleic acid Horizontal transmission mesonivirus |
Zdroj: | Viruses Volume 12 Issue 10 Viruses, Vol 12, Iss 1159, p 1159 (2020) |
ISSN: | 1999-4915 |
Popis: | The Mesoniviridae are a newly assigned family of viruses in the order Nidovirales. Unlike other nidoviruses, which include the Coronaviridae, mesoniviruses are restricted to mosquito hosts and do not infect vertebrate cells. To date there is little information on the morphological and antigenic characteristics of this new group of viruses and a dearth of mesonivirus-specific research tools. In this study we determined the genetic relationships of recent Australian isolates of Alphamesonivirus 4 (Casuarina virus&mdash CASV) and Alphamesonivirus 1 (Nam Dinh virus&mdash NDiV), obtained from multiple mosquito species. Australian isolates of NDiV showed high-level similarity to the prototype NDiV isolate from Vietnam (99% nucleotide (nt) and amino acid (aa) identity). Isolates of CASV from Central Queensland were genetically very similar to the prototype virus from Darwin (95&ndash 96% nt and 91&ndash 92% aa identity). Electron microscopy studies demonstrated that virion diameter (&asymp 80 nm) and spike length (&asymp 10 nm) were similar for both viruses. Monoclonal antibodies specific to CASV and NDiV revealed a close antigenic relationship between the two viruses with 13/34 mAbs recognising both viruses. We also detected NDiV RNA on honey-soaked nucleic acid preservation cards fed on by wild mosquitoes supporting a possible mechanism of horizontal transmission between insects in nature. |
Databáze: | OpenAIRE |
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