Mmp17b Is Essential for Proper Neural Crest Cell Migration In Vivo
| Autor: | Ramani Ramchandran, Chang Z. Chun, George A. Wilkinson, Marcus-Oliver Schupp, Ling Wang, Noah R. Leigh, Adam J. Gastonguay, Vakeel Padmanabhan, Keguo Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Morpholino
Molecular Sequence Data lcsh:Medicine Embryonic Development Matrix metalloproteinase GPI-Linked Proteins Extracellular matrix 03 medical and health sciences 0302 clinical medicine Cell Movement Animals Amino Acid Sequence lcsh:Science Zebrafish 030304 developmental biology Body Patterning 0303 health sciences Metalloproteinase Multidisciplinary biology Gene Expression Profiling lcsh:R Neural crest Cell migration Matrix Metalloproteinase 17 biology.organism_classification Cell biology Enzyme Activation Neural Crest Organ Specificity Immunology lcsh:Q Neural crest cell migration Sequence Alignment 030217 neurology & neurosurgery Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 10, p e76484 (2013) |
| ISSN: | 1932-6203 |
| Popis: | The extracellular matrix plays a critical role in neural crest (NC) cell migration. In this study, we characterize the contribution of the novel GPI-linked matrix metalloproteinase (MMP) zebrafish mmp17b. Mmp17b is expressed post-gastrulation in the developing NC. Morpholino inactivation of mmp17b function, or chemical inhibition of MMP activity results in aberrant NC cell migration with minimal change in NC proliferation or apoptosis. Intriguingly, a GPI anchored protein with metalloproteinase inhibitor properties, Reversion-inducing-Cysteine-rich protein with Kazal motifs (RECK), which has previously been implicated in NC development, is expressed in close apposition to NC cells expressing mmp17b, raising the possibility that these two gene products interact. Consistent with this possibility, embryos silenced for mmp17b show defective development of the dorsal root ganglia (DRG), a crest-derived structure affected in RECK mutant fish sensory deprived (sdp). Taken together, this study has identified the first pair of MMP, and their putative MMP inhibitor RECK that functions together in NC cell migration. |
| Databáze: | OpenAIRE |
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