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The objective of research was to isolate probiotic bacteria from catfish intestine and to examine its effects on the growth and resistance of carp against Aeromonas hydrophila. The probiotic isolated from intestine was grown on MRS agar. Fish used in this research was juvenile of carp with an average weight of 5.5 g obtained from Freshwater Aquaculture Board at Tatelu Village, North Sulawesi Province. Before running the experiment, the juveniles were adapted for one weeks in 15 glass aquaria at a density of 15 individuals each. After acclimatization, the fish were fed diet supplemented with probiotic at different concentrations namely 1x10 9 , 1x10 8 , 1x10 7 , 1x10 6 cfu/mL for three weeks. The fish were fed two times a dayat 08.00 am and 17.00 pm with a dose of 5% of body weight per day. At the end of feeding, the fish were challenged with A. hydrophila. Data collected included average growth rate, absolute growth, feed efficiency, and food conversion ratio. Research results found that the addition of probiotic into feed was able to increase growth and feed efficiency and reduce food conversion ratio as well (p |
