Genotypes Associated with Listeria monocytogenes Isolates Displaying Impaired or Enhanced Tolerances to Cold, Salt, Acid, or Desiccation Stress
| Autor: | Kevin J. Allen, Lisbeth Truelstrup Hansen, Victor P. J. Gannon, Patricia Hingston, Bhavjinder K. Dhillon, Fiona S. L. Brinkman, Chad R. Laing, Jessica C. Chen, Taurai Tasara, Claire Bertelli, Siyun Wang |
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| Přispěvatelé: | University of Zurich, Wang, Siyun |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) plasmids 030106 microbiology 610 Medicine & health Human pathogen Biology medicine.disease_cause Microbiology 2726 Microbiology (medical) 03 medical and health sciences Plasmid Listeria monocytogenes internalin A Genetic variation Genotype medicine Gene 10082 Institute of Food Safety and Hygiene 2. Zero hunger Genetics Whole genome sequencing whole genome sequencing stress tolerance 2404 Microbiology 3. Good health food safety 030104 developmental biology 570 Life sciences biology sequence typing Desiccation |
| Zdroj: | Hingston, P A, Chen, J, Dhillon, B K, Laing, C, Bertelli, C, Gannon, V, Tasara, T, Allen, K, Brinkman, F S L, Hansen, L T & Wang, S 2017, ' Genotypes Associated with Listeria monocytogenes Isolates Displaying Impaired or Enhanced Tolerances to Cold, Salt, Acid, or Desiccation Stress ', Frontiers in Microbiology, vol. 8, 369 . https://doi.org/10.3389/fmicb.2017.00369 |
| ISSN: | 1664-302X |
| Popis: | The human pathogen Listeria monocytogenes is a large concern in the food industry where its continuous detection in food products has caused a string of recalls in North America and Europe. Most recognized for its ability to grow in foods during refrigerated storage, L. monocytogenes can also tolerate several other food-related stresses with some strains possessing higher levels of tolerances than others. The objective of this study was to use a combination of phenotypic analyses and whole genome sequencing to elucidate potential relationships between L. monocytogenes genotypes and food-related stress tolerance phenotypes. To accomplish this, 166 L. monocytogenes isolates were sequenced and evaluated for their ability to grow in cold (4°C), salt (6% NaCl, 25°C), and acid (pH 5, 25°C) stress conditions as well as survive desiccation (33% RH, 20°C). The results revealed that the stress tolerance of L. monocytogenes is associated with serotype, clonal complex (CC), full length inlA profiles, and the presence of a plasmid which was identified in 55% of isolates. Isolates with full length inlA exhibited significantly (p |
| Databáze: | OpenAIRE |
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