Novel Ablation Therapy Using Endoscopic Irreversible Electroporation in the Bile Duct: A Pilot Animal Study
| Autor: | Lee, Kang Won, Lee, Jae Min, Choi, Hyuk Soon, Kim, Eun Sun, Keum, Bora, Seo, Yeon Seok, Jeen, Yoon Tae, Um, Soon Ho, Lee, Hong Sik, Chun, Hoon Jai, Kim, Chang Duck, Oh, Chi Hyuk, Kim, Hong Bae |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
endoscopic retrograde cholangiopancreatography medicine.medical_treatment Urology Medicine (miscellaneous) RC799-869 ablation Biliary disease 03 medical and health sciences 0302 clinical medicine bile duct irreversible electroporation medicine Radiology Nuclear Medicine and imaging Animal study Internal medicine Endoscopic retrograde cholangiopancreatography medicine.diagnostic_test Common bile duct Bile duct business.industry fungi Gastroenterology Irreversible electroporation Diseases of the digestive system. Gastroenterology Ablation medicine.disease RC31-1245 medicine.anatomical_structure Biliary tract 030220 oncology & carcinogenesis Original Article 030211 gastroenterology & hepatology business |
| Zdroj: | Clinical Endoscopy, Vol 54, Iss 3, Pp 413-419 (2021) Clinical Endoscopy |
| ISSN: | 2234-2443 2234-2400 |
| Popis: | Background/aims Irreversible electroporation (IRE) is a relatively new ablation method. However, the application of IRE ablation in the treatment of biliary disease has not been attempted. A minimally invasive approach using endoscopic retrograde cholangiopancreatography (ERCP) can be a novel therapeutic modality for IRE ablation. In this study, we aimed to investigate the feasibility of endoscopic IRE for the biliary tract using an animal model. Methods A new catheter-type electrode was developed for endoscopic IRE ablation of the biliary tract. We performed ERCP and endoscopic IRE ablations in the normal common bile duct of Yorkshire pigs. The experimental setting of IRE was 500 V/cm (50 pulses, 100-µs length). The animals were sacrificed after 24 hr, and the ablated bile duct was examined. Results Well-demarcated focal color changes were observed on the mucosa of the common bile duct. The depth of change after IRE was confined to the mucosal and submucosal layers. Apoptotic changes in the bile duct were observed only around the IRE ablation area. Immunohistochemistry assay showed cell death in the bile duct along the electrode. Conclusions Endoscopic IRE ablation using ERCP was successfully performed in the common bile duct. It can be a potential option for the treatment of biliary tumors. |
| Databáze: | OpenAIRE |
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