Full-Length Genomic RNA of Foot-and-Mouth Disease Virus Is Infectious for Cattle by Injection
| Autor: | Hanna Keck, Benedikt Litz, Bernd Hoffmann, Julia Sehl-Ewert, Martin Beer, Michael Eschbaumer |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Infectious Diseases
foot-and-mouth disease virus safe sample transport single-stranded positive-sense RNA TRIzol extraction naked RNA infectivity RNA transfection lipofectamine self-transfection BHK cells Foot-and-Mouth Disease Virus Virology Foot-and-Mouth Disease Animals Cattle Diseases RNA Viral Cattle Genomics virology |
| Zdroj: | Viruses; Volume 14; Issue 9; Pages: 1924 |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v14091924 |
| Popis: | Safe sample transport is of great importance for infectious diseases diagnostics. Various treatments and buffers are used to inactivate pathogens in diagnostic samples. At the same time, adequate sample preservation, particularly of nucleic acids, is essential to allow an accurate laboratory diagnosis. For viruses with single-stranded RNA genomes of positive polarity, such as foot-and-mouth disease virus (FMDV), however, naked full-length viral RNA can itself be infectious. In order to assess the risk of infection from inactivated FMDV samples, two animal experiments were performed. In the first trial, six cattle were injected with FMDV RNA (isolate A22/IRQ/24/64) into the tongue epithelium. All animals developed clinical disease within two days and FMDV was reisolated from serum and saliva samples. In the second trial, another group of six cattle was exposed to FMDV RNA by instilling it on the tongue and spraying it into the nose. The animals were observed for 10 days after exposure. All animals remained clinically unremarkable and virus isolation as well as FMDV genome detection in serum and saliva were negative. No transfection reagent was used for any of the animal inoculations. In conclusion, cattle can be infected by injection with naked FMDV RNA, but not by non-invasive exposure to the RNA. Inactivated FMDV samples that contain full-length viral RNA carry only a negligible risk of infecting animals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |